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Scanning-electron-microscope imaging of gold (electrum) nanoparticles in middle Miocene bonanza epithermal ores from northern Nevada, USA
Scanning electron microscope (SEM) imaging of high-grade gold ores from several middle Miocene bonanza epithermal Au-Ag deposits from northern NV shows that a significant amount of gold occurs as electrum nano...
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Article
New isotopic evidence bearing on bonanza (Au-Ag) epithermal ore-forming processes
New Cu, S, and Pb isotope data provide evidence for a magmatic source of metal(loid)s and sulfur in epithermal Au-Ag deposits even though their ore-forming solutions are composed primarily of heated meteoric (...
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Article
Selection of international molecular standards for DNA fingerprinting of Theobroma cacao
A collaborative international program was initiated to identify and describe the genetic diversity of living germplasm collections of Theobroma cacao genotypes that are maintained in several international collect...
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Article
A comparison of genetic relationship measures in strawberry (Fragaria × ananassa Duch.) based on AFLPs, RAPDs, and pedigree data
Nineteen of the major strawberry (Fragaria × ananassa Duch.) cultivars grown in the UnitedStates and Canada were examined for AFLP markerpolymorphisms. For the AFLP reactions, the EcoRI-ACC primer was used in com...
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Article
Modeling alternative paths of chemical evolution of Na-HCO3-type groundwater near Oak Ridge, Tennessee, USA
This paper demonstrates that both cation exchange, a commonly invoked mechanism, and silicate hydrolysis, which is less commonly considered, can produce Na-HCO3-type water in sedimentary rocks. Evolution of N...
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Article
Identification of molecular markers in soybean comparing RFLP, RAPD and AFLP DNA map** techniques
Three different DNA map** techniques—RFLP, RAPD and AFLP—were used on identical soybean germplasm to compare their ability to identify markers in the development of a genetic linkage map. Polymorphisms prese...
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Book
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Article
Rapid optimization of electroporation conditions for plant cells, protoplasts, and pollen
The optimization of electroporation conditions for maximal uptake of DNA during direct gene transfer experiments is critical to achieve high levels of gene expression in transformed plant cells. Two stains, tr...
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Article
Colorado potato beetle resistant somatic hybrid potato plants produced via protoplast electrofusion
Leptines are natural glycoalkaloids found only in certain selections of the wild potato speciesSolanum chacoense. These rare glycoalkaloids have been identified to be phytochemical defensive agents against insect...
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Protocol
Pollen Electrotransformation in Tobacco
Numerous techniques have been developed to transfer genes into plants to create genetically engineered crops that can tolerate environmental stresses, and to improve productivity and quality. The search for ea...
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Protocol
Protoplast Electrofusion and Regeneration in Potato
Protoplast fusion and subsequent in vitro plant regeneration, leading to somatic hybridization, offer opportunities for transferring entire genomes from one plant into another, regardless of the interspecific ...
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Protocol
Reporter Genes and Transient Assays for Plants
The introduction of DNA into plant cells, protoplasts, or intact tissues has been accomplished by a variety of mechanisms, including electroporation, electrofusion, particle bombardment, liposome transfer, the...
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Chapter
Successful Gene Transfer in Plants Using Electroporation and Electrofusion
Electroporation and electrofusion of plant tissues are rapid, reliable, techniques for genome modification. To accomplish these procedures two different wave forms have been developed and successfully used in ...
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Chapter
Alkaloid Chemistry and Feeding Specificity of Insect Herbivores
The role of plant secondary natural products and their interactions with insect herbivores have been focal points for research by chemists, botanists, and entomologists for several years and is the topic of se...
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Article
Expression of a foreign gene in electroporated pollen grains of tobacco
The incorporation of genetically engineered DNA into pollen and subsequent fertilization of eggs by the transformed pollen would be a convenient method for producing genetically engineered seed. This method of...
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Chapter
Gene transfer in plants
With the specter of dwindling biotic diversity, need is increased for preservation of available germplasm. Plant germplasm may be preserved in a variety of ways including seed storage, cryopreservation, and ti...
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Chapter
Plant Gene Transfer Using Electrofusion and Electroporation
In recent years, the rapidly develo** fields of electrofusion of somatic cells and electroporation of selected cell types have become an extremely important tool in the production and modification of hybrid ...
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Book
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Chapter
Electrofusion
Electric field-induced cell fusion (electrofusion) is emerging as a promising new tool in cell biology and somatic cell genetics. Compared with other cell-fusion techniques, electrofusion is rapid, simple, and...
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Chemically Induced Fusion of Plant Protoplasts
In recent years, the fusion of plant protoplasts has become an important mechanism of transferring genetic information between sexually incompatible species. The potential totipotency of plant culture systems ...
