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  1. No Access

    Article

    Clostridium sporogenes uses reductive Stickland metabolism in the gut to generate ATP and produce circulating metabolites

    Gut bacteria face a key problem in how they capture enough energy to sustain their growth and physiology. The gut bacterium Clostridium sporogenes obtains its energy by utilizing amino acids in pairs, coupling th...

    Yuanyuan Liu, Haoqing Chen, William Van Treuren, Bi-Huei Hou… in Nature Microbiology (2022)

  2. No Access

    Article

    Nectar secretion requires sucrose phosphate synthases and the sugar transporter SWEET9

    Although nectar is known to be important, for example in plant–insect interactions, little has been known about the mechanism of its secretion; sucrose phosphate synthases are now reported to be essential for ...

    I Winnie Lin, Davide Sosso, Li-Qing Chen, Klaus Gase, Sang-Gyu Kim, Danny Kessler… in Nature (2014)

  3. No Access

    Article

    Optical sensors for monitoring dynamic changes of intracellular metabolite levels in mammalian cells

    Knowledge of the in vivo levels, distribution and flux of ions and metabolites is crucial to our understanding of physiology in both healthy and diseased states. The quantitative analysis of the dynamics of ions ...

    Bi-Huei Hou, Hitomi Takanaga, Guido Grossmann, Li-Qing Chen… in Nature Protocols (2011)

  4. No Access

    Article

    Sugar transporters for intercellular exchange and nutrition of pathogens

    Sugar efflux transporters are essential for the maintenance of animal blood glucose levels, plant nectar production, and plant seed and pollen development. Despite broad biological importance, the identity of ...

    Li-Qing Chen, Bi-Huei Hou, Sylvie Lalonde, Hitomi Takanaga, Mara L. Hartung… in Nature (2010)

  5. No Access

    Article

    Rapid optimization of electroporation conditions for plant cells, protoplasts, and pollen

    The optimization of electroporation conditions for maximal uptake of DNA during direct gene transfer experiments is critical to achieve high levels of gene expression in transformed plant cells. Two stains, tr...

    James A. Saunders, Chin Ho Lin, Bi Huei Hou, Jian** Cheng… in Molecular Biotechnology (1995)