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Article
The occurrence of phenotypically complementary apomixis-recombinants in crosses between sexual and apomictic dandelions (Taraxacum officinale)
Apomictic seed development in dandelion (Taraxacum officinale) involves (1) restitutional meiosis (diplospory), (2) egg cell parthenogenesis, and (3) autonomous endosperm development. The question is whether thes...
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Article
Chromosome arrangement and behaviour of two rye homologous telosomes at the onset of meiosis in disomic wheat-5RL addition lines with and without the Ph1 locus
Fluorescence in-situ hybridization (FISH) of total genomic and repetitive DNA on microsporocytes of ditelocentric addition lines of rye 5RL in hexaploid wheat was performed to study the behaviour of the rye homol...
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Article
Visualization of the terminal structure of rice chromosomes 6 and 12 with multicolor FISH to chromosomes and extended DNA fibers
High-resolution fluorescence in situ hybridization (FISH) on interphase and pachytene nuclei, and extended DNA fibers enabled microscopic distinction of DNA sequences less than a few thousands of base pairs apart...
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Article
Chromosome painting in plants
The current 'state-of-art' as to chromosome painting in plants is reviewed. We define different situations described as painting so far: i) Genomic in situ hybridisation (GISH) with total genomic DNA to distingui...
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Chapter
Chromosome painting in plants
The current ‘state-of-art’ as to chromosome painting in plants is reviewed. We define different situations described as painting so far: i) Genomic in situ hybridisation (GISH) with total genomic DNA to distingui...
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Article
Cre/lox-mediated recombination in Arabidopsis: evidence for transmission of a translocation and a deletion event
Cre recombinase was used to mediate recombination between a chromosomally introduced loxP sequence in Arabidopsis thaliana (35S-lox-cre) and transferred DNA (T-DNA) originating from Agrobacterium tumefaciens (plo...
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Article
FISH to mitotic chromosomes and extended DNA fibres of Beta procumbens in a series of monosomic additions to beet (B. vulgaris)
The physical localization and organization of a Procumbentes-specific repetitive DNA sequence, PB6-4, on the chromosomes of Beta procumbens (2n=18) were studied, using FISH (fluorescence in situ hybridization) to...
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Article
Introgression of Lilium Rubellum Baker Chromosomes into L. Longiflorum Thunb.: A Genome Painting Study of the F1 Hybrid, BC1 and BC2 Progenies
Interspecific hybrids between Lilium longiflorum (L, 2n=2x=24) andLilium rubellum (R, 2n=2x=24) were produced with the aim of transferring desirable horticultural traits from L. rubellum to L. longiflorum. All F1
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Article
The effect of the wheat Ph1 locus on chromatin organisation and meiotic chromosome pairing analysed by genome painting
The Ph1 locus in wheat influences homo(eo)logous chromosome pairing. We have analysed its effect on the behaviour and morphology of two 5RL rye telosomes in a wheat background, by genomic in situ hybridisation (...
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Article
Molecular cytogenetics of Alstroemeria: identification of parental genomes in interspecific hybrids and characterization of repetitive DNA families in constitutive heterochromatin
The genus Alstroemeria consists of diploid(2n=2x=16) species originating mainly from Chile and Brazil. Most cultivars are triploid or tetraploid interspecific hybrids. C-banding of eight species revealed obvio...
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Article
High-resolution map** on pachytene chromosomes and extended DNA fibres by fluorescencein-situ hybridisation
This article describes two protocols for high-resolution physical map** of DNA sequences in tomato using fluorescencein situ hybridisation (FISH). The first technique involves FISH to spread chromosomes from po...
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Article
Preparation of tomato meiotic pachytene and mitotic metaphase chromosomes suitable for fluorescencein situ hybridization (FISH)
Fluorescencein situ hybridization (FISH) is an increasingly powerful tool with a variety of applications in both basic and applied research. With excellent genetic, cytogenetic and molecular maps available, the t...