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Article
Open AccessThe construction and use of bacterial DNA microarrays based on an optimized two-stage PCR strategy
DNA microarrays are a powerful tool with important applications such as global gene expression profiling. Construction of bacterial DNA microarrays from genomic sequence data using a two-stage PCR amplificatio...
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Article
Expression and functional analysis of the rice plasma-membrane intrinsic protein gene family
Plasma membrane intrinsic proteins (PIPs) are a subfamily of aquaporins that enable fast and controlled translocation of water across the membrane. In this study, we systematically identified and cloned ten PIP g...
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Article
Open AccessIn silico and microarray-based genomic approaches to identifying potential vaccine candidates against Leptospira interrogans
Currently available vaccines against leptospirosis are of low efficacy, have an unacceptable side-effect profile, do not induce long-term protection, and provide no cross-protection against the different serov...
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Article
AtTHIC, a gene involved in thiamine biosynthesis in Arabidopsis thaliana
Thiamine (vitamin B1) is an essential compound for organisms. It contains a pyrimidine ring structure and a thiazole ring structure. These two moieties of thiamine are synthesized independently and then coupled t...
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Article
GIDE is a mitochondrial E3 ubiquitin ligase that induces apoptosis and slows growth
Here, we report the identification of GIDE, a mitochondrially located E3 ubiquitin ligase. GIDE contains a C-terminal RING finger domain, which is mostly conserved with those of the IAP family members and is r...
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Article
Velleratretraol, an unusual highly functionalized lactarane sesquiterpene from Lactarius vellereus
An unusual highly functionalized lactarane sesquiterpene, named velleratretraol (1), was isolated from the ethanol extract of the fruiting body of the mushroom Lactarius vellereus. Its structure was determined th...
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Article
Open AccessIdentification and functional characterization of small non-coding RNAs in Xanthomonas oryzae pathovar oryzae
Small non-coding RNAs (sRNAs) are regarded as important regulators in prokaryotes and play essential roles in diverse cellular processes. Xanthomonas oryzae pathovar oryzae (Xoo) is an important plant pathogenic ...
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Article
Conformational conversion and prion disease
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Article
Open AccessHypoxia-mimetic agents inhibit proliferation and alter the morphology of human umbilical cord-derived mesenchymal stem cells
The therapeutic efficacy of human mesenchymal stem cells (hMSCs) for the treatment of hypoxic-ischemic diseases is closely related to level of hypoxia in the damaged tissues. To elucidate the potential therape...
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Article
Open AccessExpansion and diversification of the SET domain gene family following whole-genome duplications in Populus trichocarpa
Histone lysine methylation modifies chromatin structure and regulates eukaryotic gene transcription and a variety of developmental and physiological processes. SET domain proteins are lysine methyltransferases...
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Article
Piglets cloned from induced pluripotent stem cells
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Open AccessThe genome- and transcriptome-wide analysis of innate immunity in the brown planthopper, Nilaparvata lugens
The brown planthopper (Nilaparvata lugens) is one of the most serious rice plant pests in Asia. N. lugens causes extensive rice damage by sucking rice phloem sap, which results in stunted plant growth and the tra...
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Article
Open AccessGlobal transcriptome profiles of Camellia sinensis during cold acclimation
Tea is the most popular non-alcoholic health beverage in the world. The tea plant (Camellia sinensis (L.) O. Kuntze) needs to undergo a cold acclimation process to enhance its freezing tolerance in winter. Change...
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Open AccessComparative transcriptome analysis of tomato (Solanum lycopersicum) in response to exogenous abscisic acid
Abscisic acid (ABA) can regulate the expressions of many stress-responsive genes in plants. However, in defense responses to pathogens, mounting evidence suggests that ABA plays variable roles. Little informat...
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Molecular cloning, genomic structure, polymorphism and expression analysis of major histocompatibility complex class IIA gene of swamp eel Monopterus albus
Major histocompatibility complex (MHC) class II molecules play an important role in the immune response of vertebrates. In this paper, full-length MHC IIA cDNA was isolated from swamp eel (Monopterus albus) by ra...
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Open AccessA phylogeny for the pomatiopsidae (Gastropoda: Rissooidea): a resource for taxonomic, parasitological and biodiversity studies
The Pomatiopsidae are reported from northern India into southern China and Southeast Asia, with two sub-families, the Pomatiopsinae (which include freshwater, amphibious, terrestrial and marine species) and th...
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Article
Open AccessSequence analysis of mitochondrial ND1 gene can reveal the genetic structure and origin of Bactrocera dorsalis s.s.
The oriental fruit fly, Bactrocera dorsalis s.s., is one of the most important quarantine pests in many countries, including China. Although the oriental fruit fly has been investigated extensively, its origins a...
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Article
Tom70 serves as a molecular switch to determine pathological cardiac hypertrophy
Pathological cardiac hypertrophy is an inevitable forerunner of heart failure. Regardless of the etiology of cardiac hypertrophy, cardiomyocyte mitochondrial alterations are always observed in this context. Th...
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Article
Open AccessThe catalytic region and PEST domain of PTPN18 distinctly regulate the HER2 phosphorylation and ubiquitination barcodes
The tyrosine phosphorylation barcode encoded in C-terminus of HER2 and its ubiquitination regulate diverse HER2 functions. PTPN18 was reported as a HER2 phosphatase; however, the exact mechanism by which it de...
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Article
Open AccessAltered proteostasis in aging and heat shock response in C. elegans revealed by analysis of the global and de novo synthesized proteome
Protein misfolding and aggregation as a consequence of impaired protein homeostasis (proteostasis) not only characterizes numerous age-related diseases but also the aging process itself. Functionally related t...