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Open AccessTHOUSAND-GRAIN WEIGHT 6, which is an IAA-glucose hydrolase, preferentially recognizes the structure of the indole ring
An indole-3-acetic acid (IAA)-glucose hydrolase, THOUSAND-GRAIN WEIGHT 6 (TGW6), negatively regulates the grain weight in rice. TGW6 has been used as a target for breeding increased rice yield. Moreover, the a...
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Article
Open AccessStructures of a FtsZ single protofilament and a double-helical tube in complex with a monobody
FtsZ polymerizes into protofilaments to form the Z-ring that acts as a scaffold for accessory proteins during cell division. Structures of FtsZ have been previously solved, but detailed mechanistic insights ar...
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Article
Open AccessC9orf72-derived arginine-rich poly-dipeptides impede phase modifiers
Nuclear import receptors (NIRs) not only transport RNA-binding proteins (RBPs) but also modify phase transitions of RBPs by recognizing nuclear localization signals (NLSs). Toxic arginine-rich poly-dipeptides ...
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Article
Open AccessStructure–function studies of ultrahigh molecular weight isoprenes provide key insights into their biosynthesis
Some plant trans-1,4-prenyltransferases (TPTs) produce ultrahigh molecular weight trans-1,4-polyisoprene (TPI) with a molecular weight of over 1.0 million. Although plant-derived TPI has been utilized in various ...
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Article
Open AccessMechanism of karyopherin-β2 binding and nuclear import of ALS variants FUS(P525L) and FUS(R495X)
Mutations in the RNA-binding protein FUS cause familial amyotropic lateral sclerosis (ALS). Several mutations that affect the proline-tyrosine nuclear localization signal (PY-NLS) of FUS cause severe juvenile ...
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Article
Open AccessStructure-Guided Design of a Fluorescent Probe for the Visualization of FtsZ in Clinically Important Gram-Positive and Gram-Negative Bacterial Pathogens
Addressing the growing problem of antibiotic resistance requires the development of new drugs with novel antibacterial targets. FtsZ has been identified as an appealing new target for antibacterial agents. Her...
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Article
Localization and expression pattern of amelotin, odontogenic ameloblast-associated protein and follicular dendritic cell-secreted protein in the junctional epithelium of inflamed gingiva
The purpose of this study is to elucidate the localization of amelotin (AMTN), odontogenic ameloblast-associated protein (ODAM) and follicular dendritic cell-secreted protein (FDC-SP) at the junctional epithel...
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Article
Open AccessA RuBisCO-mediated carbon metabolic pathway in methanogenic archaea
Two enzymes are considered to be unique to the photosynthetic Calvin–Benson cycle: ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), responsible for CO2 fixation, and phosphoribulokinase (PRK). Some arch...
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Article
Promotion of protein crystal growth by actively switching crystal growth mode via femtosecond laser ablation
The protein crystal growth mechanism can be changed from planar 2D nucleation growth to spiral growth by femtosecond laser ablation. By using this method, the growth rate of a hen egg-white lysozyme crystal in...
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Article
Amelotin gene expression is temporarily being upregulated at the initiation of apoptosis induced by TGFβ1 in mouse gingival epithelial cells
Amelotin (AMTN) is expressed and secreted by ameloblasts in the maturation stage of amelogenesis and persist with low levels in the junctional epithelium (JE) of erupted teeth. The purpose of this study is to ...
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Article
Effects of interleukin-11 on the expression of human bone sialoprotein gene
Interleukin-11 (IL-11) is a bone marrow stromal fibroblast-derived cytokine with a wide spectrum of activities in different biological systems. IL-11 and IL-6 are two cytokines known to rely on osteoblast–oste...
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Article
Laser energy dependence on femtosecond laser-induced nucleation of protein
We have investigated femtosecond laser-induced nucleation of hen egg-white lysozyme (HEWL) as a function of the laser pulse energy and pulse time width. This is the first recorded study to confirm that the fem...
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Article
Erratum: Mechanism of metal activation of human hematopoietic prostaglandin D synthase
Nat. Struct. Biol. 10, 291–296 (2003). The y-axes of Fig. 3a,b,d in this manuscript were labeled incorrectly. The correct unit for the PDGS activity in these panels should be μmol min−1 mg−1 protein. Additiona...
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Article
Mechanism of metal activation of human hematopoietic prostaglandin D synthase
Here we report the crystal structures of human hematopoietic prostaglandin (PG) D synthase bound to glutathione (GSH) and Ca2+ or Mg2+. Using GSH as a cofactor, prostaglandin D synthase catalyzes the isomerizatio...
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Three-Dimensional Structure of Phosphoenolpyruvate Carboxylase from Escherichia coli at 2.8Å Resolution
Phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) irreversibly catalyzes the carboxylation of phosphoenolpyruvate to form oxaloacetate and inorganic phosphate using Mg2+ as a cofactor.