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Article
Open AccessCXCL5 activates CXCR2 in nociceptive sensory neurons to drive joint pain and inflammation in experimental gouty arthritis
Gouty arthritis evokes joint pain and inflammation. Mechanisms driving gout pain and inflammation remain incompletely understood. Here we show that CXCL5 activates CXCR2 expressed on nociceptive sensory neuron...
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Article
Open AccessAnoctamin 1 controls bone resorption by coupling Cl− channel activation with RANKL-RANK signaling transduction
Osteoclast over-activation leads to bone loss and chloride homeostasis is fundamental importance for osteoclast function. The calcium-activated chloride channel Anoctamin 1 (also known as TMEM16A) is an import...
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Article
Emerging Modulators of TMEM16A and Their Therapeutic Potential
Calcium-activated chloride channels (CaCCs) are widespread chloride channels which rely on calcium activation to perform their functions. In 2008, TMEM16A (also known as anoctamin1, ANO1) was identified as the...
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Article
Molecular Mechanisms and Structural Basis of Retigabine Analogues in Regulating KCNQ2 Channel
KCNQ2 channel is one of the important members of potassium voltage-gated channel. KCNQ2 is closely related to neuronal excitatory diseases including epilepsy and neuropathic pain, and also acts as a drug targe...
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Article
Co-culturing on dry filter paper significantly increased the efficiency of Agrobacterium-mediated transformations of maize immature embryos
In the Agrobacterium tumefaciens-mediated transformations of maize immature embryos (IEs), the common co-culturing media used are MS or N6-based (MC). Here, we used a novel co-culturing method in which maize ‘Qi3...
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Article
Open AccessDivergent roles of FT-like 9 in flowering transition under different day lengths in Brachypodium distachyon
Timing of reproductive transition is precisely modulated by environmental cues in flowering plants. Facultative long-day plants, including Arabidopsis and temperate grasses, trigger rapid flowering in long-day...
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Article
The Natural Compound Cinnamaldehyde is a Novel Activator of Calcium-Activated Chloride Channel
Calcium-activated chloride channels (CaCCs) play important roles in a multitude of physiological processes, and in many cells types, TMEM16A was identified as the molecular basis of CaCC. Abnormal CaCC functio...
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Article
Hydrocinnamic Acid Inhibits the Currents of WT and SQT3 Syndrome-Related Mutants of Kir2.1 Channel
Gain of function in mutations, D172N and E299V, of Kir2.1 will induce type III short QT syndrome. In our previous work, we had identified that a mixture of traditional Chinese medicine, styrax, is a blocker of...
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Article
Identification of Resveratrol, an Herbal Compound, as an Activator of the Calcium-Activated Chloride Channel, TMEM16A
Calcium-activated chloride channels (CaCCs) play vital roles in a variety of physiological processes. Dysfunction of the CaCCs is implicated in many diseases. Drug discovery targeting at CaCCs has recently bec...
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Article
Ginsenoside Rb1, a novel activator of the TMEM16A chloride channel, augments the contraction of guinea pig ileum
Calcium-activated chloride channels (CaCCs) play important roles in many physiological processes, and the molecular basis of CaCCs has been identified as TMEM16A in many cell types. It is well established that...
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Article
Open AccessTree-ring widths are good proxies of annual variation in forest productivity in temperate forests
Tree rings have long been used to calibrate the net primary production (NPP) time-series predicted by process-based models, based on an implicit assumption that ring-width indices (RWI) can well reflect tempor...
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Article
Eag1 Voltage-Dependent Potassium Channels: Structure, Electrophysiological Characteristics, and Function in Cancer
Eag1 (ether-à-go-go-1), a member of the voltage-dependent potassium channel family, is expressed mainly in the brain, and at low levels in placenta, testis, and adrenal gland, and only transiently in myoblasts...
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Article
Characterization of PM2.5-bound polycyclic aromatic hydrocarbons and its deposition in Populus tomentosa leaves in Bei**g
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous constituents of air particulate matter and can be taken up by plants from the atmosphere. However, the purification of particulate-bound PAHs in the atmos...
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Article
15N-labeled ammonium nitrogen uptake and physiological responses of poplar exposed to PM2.5 particles
Air pollution caused by particulate matter with aerodynamic diameters less than 2.5 μm (PM2.5) is a serious environmental problem. Plants can improve air quality by removing PM2.5 from the atmosphere. However, di...
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Article
Open AccessStructural Basis for Differences in Dynamics Induced by Leu Versus Ile Residues in the CD Loop of Kir Channels
The effect of the conserved Leu/Ile site in the CD loop on the gating dynamics of Kir channels and corresponding micro-structural mechanism remains unclear. Molecular dynamics simulations were performed to inv...
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Article
Two Ca2+-Binding Sites Cooperatively Couple Together in TMEM16A Channel
TMEM16A is the molecular basis of calcium-activated chloride channels and shows Ca2+-dependent gating. It is critical to understand how the Ca2+ sensors dynamically control the gate of TMEM16A. However, the detai...
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Article
Open AccessIdentification of the Conformational transition pathway in PIP2 Opening Kir Channels
The gating of Kir channels depends critically on phosphatidylinositol 4,5-bisphosphate (PIP2), but the detailed mechanism by which PIP2 regulates Kir channels remains obscure. Here, we performed a series of Targe...
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Article
Divalent Cations Modulate TMEM16A Calcium-Activated Chloride Channels by a Common Mechanism
The gating of Ca2+-activated Cl− channels is controlled by a complex interplay among [Ca2+]i, membrane potential and permeant anions. Besides Ca2+, Ba2+ also can activate both TMEM16A and TMEM16B. This study repo...
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Article
Combining fragment homology modeling with molecular dynamics aims at prediction of Ca2+ binding sites in CaBPs
The family of calcium-binding proteins (CaBPs) consists of dozens of members and contributes to all aspects of the cell’s function, from homeostasis to learning and memory. However, the Ca2+-binding mechanism is ...
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Article
Open AccessGhMPK16, a novel stress-responsive group D MAPK gene from cotton, is involved in disease resistance and drought sensitivity
Mitogen-activated protein kinase (MAPK) cascades play pivotal roles in mediating biotic and abiotic stress responses. In plants, MAPKs are classified into four major groups (A-D) according to their sequence ho...
