17 Result(s)

Protocol

Screening for MAPK Modulators Using an In-Cell Western Assay

Several essential cellular functions are critically influenced by the Ras-Raf-MEK-ERK cascade, and pathological problems have been implicated with loss as well as gain of its activity. Therefore, there is a gr...

Simon Schnaiter, Beatrix Fürst, Johannes Neu, Frigyes Wáczek, László Őrfi… in Ras Signaling (2014)

Article

Integrating molecular diagnostics into anticancer drug discovery

Efforts to repeat the success of pioneering molecularly targeted cancer drugs, such as trastuzumab, for particular patient populations have been hampered by factors such as a lack of correlation between the mo...

István Peták, Richárd Schwab, László Őrfi, László Kopper… in Nature Reviews Drug Discovery (2010)

Article

Proteomics strategy for quantitative protein interaction profiling in cell extracts

Quantitative information is necessary to determine which protein interactions are the most relevant in a cellular context. A defined set of affinity purification experiments combined with quantitative mass spe...

Kirti Sharma, Christoph Weber, Michaela Bairlein, Zoltán Greff… in Nature Methods (2009)

Article

Binding specificity of the l-arginine transport systems in mouse macrophages and human cells overexpressing the cationic amino acid transporter hCAT-1

The uptake of l-arginine into mouse peritoneal macrophages can be inhibited by numerous amino acids and derivatives. Kinetic studies showed an almost entirely competitive inhibition for both cationic and neutral ...

Dániel Erős, László Őrfi, Ildikó Csuka, György Kéri, András Hrabák in Amino Acids (2009)

Article

Validation subset selections for extrapolation oriented QSPAR models

One of the most important features of QSPAR models is their predictive ability. The predictive ability of QSPAR models should be checked by external validation. In this work we examined three different types o...

Csaba Szántai-Kis, István Kövesdi, György Kéri, László Örfi in Molecular Diversity (2003)

Article

Pharmacological characterisation of the somatostatin analogue TT-232: effects on neurogenic and non-neurogenic inflammation and neuropathic hyperalgesia

The putative anti-inflammatory and anti-nociceptive activity of the heptapeptide somatostatin analogue TT-232 (D-Phe-Cys-Tyr-D-Thr-Lys-Cys-Thr-NH₂) was investigated in the rat and mouse, as well as its effect on ...

Erika Pintér, Zsuzsanna Helyes… in Naunyn-Schmiedeberg's Archives of Pharmaco… (2002)

Chapter

Peptidomimetic tyrosine kinase inhibitors induce non apoptotic, Clarke-III type programmed cell death

György Kéri, László Ôrfi, Gergely Szántó… in Peptides Frontiers of Peptide Science (2002)

Chapter

Further Studies of the Signaling Mechanisms of the Antitumor Somatostatin Analogue TT-232

The somatostatin structural analogue TT-232 is a heptapeptide of a cyclopenta-ring structure: D-Phe-Cys-Tyr-D-Thr-Lys-Cys-Thr-NH₂ that was shown to have strong antiproliferative and apoptotic effects on tumor cel...

Attila Steták, Tibor Vántus, Gyöngyi Bökönyi… in Peptides: The Wave of the Future (2001)

Chapter

New Antiproliferative Agents from a Peptidomimetic Library

It is known that chiral ATP analogue quinazolines show selective Epidermal Growth Factor Receptor Tyrosine Kinase (EGFR TK) inhibitory activity depending on the chirality of the molecule [1]. We have developed...

László Őrfi, Györgyi Bökönyi, Anikó Horváth… in Peptides: The Wave of the Future (2001)

Chapter

HPLC Analysis, Modeling, and Biological Studies of Antiproliferative Heterocyclic Carboxamides

Reversed phase HPLC and MEKC [1] provide excellent possibilities to measure the hydrophobic properties of compounds, characterizing them directly from the chromatographic retention time. The aim in this respec...

János Seprõdi, Ferenc Hollósy, Dániel Erõs, László Õrfi… in Peptides: The Wave of the Future (2001)

Article

New antitumor leads from a peptidomimetic library

A parallel combinatorial library of over 1600 compounds has been designed and synthesized for the development of new potential peptidomimetic protein tyrosine kinase (PTK) inhibitor leads. These peptidomimetic...

László Õrfi, Frigyes Wáczek, István Kövesdi, György Mészáros… in Letters in Peptide Science (1999)

Article

New antitumor leads from a peptidomimetic library

A parallel combinatorial library of over 1600 compounds has been designed and synthesized for the development of new potential peptidomimetic protein tyrosine kinase (PTK) inhibitor leads. These peptidomimetic...

László Õrfi, Frigyes Wáczek, István Kövesdi, György Mészáros… in Letters in Peptide Science (1999)

Chapter

Biodegradation of Luteinizing Hormone-Releasing Hormone

The concept that the proteolytic degradation of peptide hormones could be one of the key mechanisms regulating the homeostasis of the hormone, as proposed by Knights et al. (1973), is widely accepted. However, cu...

Károly Nikolics, György Kéri, Balázs Szöke… in Hormonally Active Brain Peptides (1982)

Chapter

Tritium Labeling of Brain Peptides

During the past decade, radiolabeling of biologically active peptides has become a widely applied and essential technique for a variety of biochemical and physiological investigations.

István Teplán, Imre Mező, Károly Nikolics… in Hormonally Active Brain Peptides (1982)