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Article
Expression profiling of extramedullary acute myeloid leukemia suggests involvement of epithelial–mesenchymal transition pathways
Extramedullary (EM) colonization is a rare complication of acute myeloid leukemia (AML), occurring in about 10% of patients, but the processes underlying tissue invasion are not entirely characterized. Through...
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Article
Effects of intermittent parathyroid hormone (PTH) administration on SOST mRNA and protein in rat bone
Sclerostin, the secreted protein product of the SOST gene, which is mainly expressed by osteocytes, has recently been proposed as a negative regulator of bone osteoblastogenesis. Chronic elevation of PTH reduc...
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Article
The presence of different growth factors does not influence bone response to hydroxyapatite: preliminary results
Porous, non-resorbable, granular hydroxyapatite (pHA), either pure or mixed with fibrin, or soaked in solutions of the growth factors PDGF, TGF, IGF, or TNF, has been implanted in rabbits under the derma and p...
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Article
Large-Eddy Simulations of Shear Flows
The general framework of large-eddy simulations (LES) is presented first, with Smagorinsky's model. Afterwards, Kraichnan's spectral eddy-viscosity is introduced and it is shown how it can be handled for LES p...
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Article
Matrix vesicles and focal proteoglycan aggregates are the nucleation sites revealed by the lanthanum incubation method: A correlated study on the hypertrophic zone of the rat epiphyseal cartilage
Correlated studies were performed with light and electron microscopy, and backscattered electron image in conjunction with X-ray microanalysis, of lanthanum-incubated epiphyseal cartilage of the young rat. The...
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Article
Influence of specimen preparation on the identification of phospholipids by the phospholipase A2-gold method in mineralizing cartilage and bone
The role of phospholipids in biological mineralization has been hypothesized but not fully elucidated. In order to identify phospholipids at the ultrastructural level in the mineralizing extracellular matrix, ...
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Article
The anatomy of bone sialoprotein immunoreactive sites in bone as revealed by combined ultrastructural histochemistry and immunohistochemistry
Bone sialoprotein was immunolocalized at the EM level in thin Lowicryl K4M sections of rat bone. Because of the unconventional EM morphology of the bone matrix seen in thin demineralized acrylate sections, the...
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Article
Culture and differentiation of chondrocytes entrapped in alginate gels
We studied the response to culture conditions and the differentiative ability in suspension culture in alginate gels of resting chondrocytes from the preosseous cartilage of adult pig scapula. It was found tha...
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Article
Extracellular alkaline phosphatase activity in mineralizing matrices of cartilage and bone: ultrastructural localization using a cerium-based method
The ultrastructural localization of alkaline phosphatase (AlP) activity has been demonstrated in epiphyseal growth cartilage and metaphyseal bone of rats. Epiphyso-metaphyseal specimens were decalcified with E...
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Article
Immunohistochemical localization of osteonectin in develo** human and calf bone using monoclonal antibodies
Osteonectin was immunolocalized in human fetal and calf neonatal develo** bone using newly developed monoclonal antibodies. The protein was localized to the cytoplasm of osteoblasts and young osteocytes. In ...
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Article
Osteonectin and GLA-Protein in calf bone: Ultrastructural immunohistochemical localization using the protein A-gold method
Osteonectin and bone Gla-protein have been localized at the ultrastructural level in calf scapula bone tissue. Post-embedding-decalcified thin sections of paraformaldehyde-fixed and Araldite-embedded specimens...
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Ultrastructural Study of Bone and Small Intestine Cells in Lead-Intoxicated Rats
Lead poisoning is one of the oldest known intoxications and is rather frequent also in our modern era, chiefly because lead use in industrial processes has increased. Lead encephalopathy, peripheral neuritis, ...
