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Immunohistochemical localization of osteonectin in develo** human and calf bone using monoclonal antibodies

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Summary

Osteonectin was immunolocalized in human fetal and calf neonatal develo** bone using newly developed monoclonal antibodies. The protein was localized to the cytoplasm of osteoblasts and young osteocytes. In bone matrix, strong reactivity was found in newly laid down osteoid. Bone matrix immunoreactivity was enhanced by pretreatment of sections with proteases, possibly because of an unmasking of epitopes engaged in protein-protein interactions. Osteonectin immuno-reactivity was also found in preosteoblasts in all types of human fetal osteogenesis (membranous, endochondral, subperiosteal, and mantellar (Meckel's cartilage) ossification), and in some chondrocytes of metaphyseal growth plate, posibly modulating towards an osteoblastic phenotype.

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Authors and Affiliations

  1. Department of Human Biopathology, Division of Pathology, La Sapienza University, Rome, Italy

    P. Bianco, G. Silvestrini & E. Bonucci

  2. National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland, USA

    J. D. Termine

Authors
  1. P. Bianco
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  2. G. Silvestrini
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  3. J. D. Termine
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  4. E. Bonucci
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Bianco, P., Silvestrini, G., Termine, J.D. et al. Immunohistochemical localization of osteonectin in develo** human and calf bone using monoclonal antibodies. Calcif Tissue Int 43, 155–161 (1988). https://doi.org/10.1007/BF02571313

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  • DOI: https://doi.org/10.1007/BF02571313

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