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Article
Open AccessRegulation of cyclin T1 during HIV replication and latency establishment in human memory CD4 T cells
The regulatory cyclin, Cyclin T1 (CycT1), is a host factor essential for HIV-1 replication in CD4 T cells and macrophages. The importance of CycT1 and the Positive Transcription Elongation Factor b (P-TEFb) co...
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Article
HIV Persistence in Adipose Tissue Reservoirs
The purpose of this review is to examine the evidence describing adipose tissue as a reservoir for HIV-1 and how this often expansive anatomic compartment contributes to HIV persistence.
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Article
Open AccessHIV-1 viral protein R (Vpr) induces fatty liver in mice via LXRα and PPARα dysregulation: implications for HIV-specific pathogenesis of NAFLD
HIV patients develop hepatic steatosis. We investigated hepatic steatosis in transgenic mice expressing the HIV-1 accessory protein Vpr (Vpr-Tg) in liver and adipose tissues, and WT mice infused with synthetic...
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Article
Open AccessInfectious SIV resides in adipose tissue and induces metabolic defects in chronically infected rhesus macaques
HIV reservoirs pose major challenges to viral eradication. The main cellular reservoirs include CD4 T cells and macrophages, whereas anatomic reservoirs are thought to be primarily lymphoid tissues. Adipose ti...
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Article
Open AccessGranzyme B secretion by human memory CD4 T cells is less strictly regulated compared to memory CD8 T cells
Granzyme B (GrzB) is a serine proteinase expressed by memory T cells and NK cells. Methods to measure GrzB protein usually involve intracellular (flow cytometry) and extracellular (ELISA and ELISpot) assays. C...
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Article
Open AccessRelationship of ethnicity and CD4 Count with glucose metabolism among HIV patients on Highly-Active Antiretroviral Therapy (HAART)
HIV patients on HAART are prone to metabolic abnormalities, including insulin resistance, lipodystrophy and diabetes. This study purports to investigate the relationship of ethnicity and CD4+ T cell count atta...
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Protocol
IL-7 Addition Increases Spot Size and Number as Measured by T-SPOT.TB ®
The interferon-gamma (IFN-γ) release assay (IGRA) is an in vitro extension of the century-old in vivo tuberculin skin test, better known as the TST. Shortcomings to the TST are multifactorial and include limit...
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Article
Detrimental effects of adenosine signaling in sickle cell disease
Yu** Zhang et al. discovered that the concentration of adenosine in the blood is increased both in a mouse model of sickle cell disease and in humans with this disease. Adenosine seems to have a pathological rol...
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Article
Open AccessCD8 apoptosis may be a predictor of T cell number normalization after immune reconstitution in HIV
As part of the Houston Vanguard study, a subset of 10 patients randomized to receive IL-2 therapy were compared to 4 patients randomized to not receive IL-2, for markers of T cell activation and death during t...
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Protocol
Prenatal Diagnosis of Chromosomal Abnormalities Using Maternal Blood
Fetal chromosomal aneuploidies can be detected by fluorescent in situ hybridization (FISH) analysis of intact fetal cells circulating in maternal blood. Given the low number of circulating fetal cells (1 in 104 t...
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Article
Epstein-Barr Virus-Transformed B-Cell Line (DV-1) Derived from Bone Marrow of a Patient with Severe Combined Immunodeficiency and Immunoblastic Lymphoma
ABSTRACT: An Epstein-Barr virus-transformed B-cell line derived from a patient with severe combined immunodeficiency who died of a lymphoreticular malignancy has been characterized. The line derived from bone ...
