-
Article
Open AccessThe chromatin landscape of healthy and injured cell types in the human kidney
There is a need to define regions of gene activation or repression that control human kidney cells in states of health, injury, and repair to understand the molecular pathogenesis of kidney disease and design ...
-
Article
Open AccessAn atlas of healthy and injured cell states and niches in the human kidney
Understanding kidney disease relies on defining the complexity of cell types and states, their associated molecular profiles and interactions within tissue neighbourhoods1. Here we applied multiple single-cell an...
-
Article
Open AccessAuthor Correction: Comparative cellular analysis of motor cortex in human, marmoset and mouse
-
Article
Scalable dual-omics profiling with single-nucleus chromatin accessibility and mRNA expression sequencing 2 (SNARE-seq2)
Comprehensive characterization of cellular heterogeneity and the underlying regulatory landscapes of tissues and organs requires a highly robust and scalable method to acquire matched RNA and chromatin accessi...
-
Article
Open AccessComparative cellular analysis of motor cortex in human, marmoset and mouse
The primary motor cortex (M1) is essential for voluntary fine-motor control and is functionally conserved across mammals1. Here, using high-throughput transcriptomic and epigenomic profiling of more than 450,000 ...
-
Article
Open AccessA perceptual scaling approach to eyewitness identification
Eyewitness misidentification accounts for 70% of verified erroneous convictions. To address this alarming phenomenon, research has focused on factors that influence likelihood of correct identification, such a...
-
Article
Reply to ‘DNA methylation haplotypes as cancer markers’
-
Protocol
Large-Scale Targeted DNA Methylation Analysis Using Bisulfite Padlock Probes
Bisulfite padlock probes (BSPP) are a method for the targeted quantification of DNA methylation in mammalian genomes. They can simultaneously characterize the level of methylcytosine modification in a large nu...
-
Article
Identification of methylation haplotype blocks aids in deconvolution of heterogeneous tissue samples and tumor tissue-of-origin map** from plasma DNA
Kun Zhang and colleagues present a metric called methylation haplotype load (MHL) that quantifies methylation patterns within blocks of tightly linked CpG dinucleotides. They show that the MHL can distinguish ...
-
Article
Open AccessGenome-wide analysis reveals TET-and TDG-mediated 5-methylcytosine oxidation dynamics
-
Article
Open AccessGenome-wide analysis reveals TET-and TDG-mediated 5-methylcytosine oxidation dynamics
-
Article
Open Access5mC and 5hmC dynamics during PGC reprogramming and role of Tet1 in female meiosis
-
Article
Tet1 controls meiosis by regulating meiotic gene expression
A loss-of-function approach in mice is used to show that the methylcytosine dioxygenase Tet1 has a role in regulating meiosis and meiotic gene activation in female germ cells; Tet1 deficiency does not greatly ...
-
Article
Library-free methylation sequencing with bisulfite padlock probes
The pairing of bisulfite padlock probes with a probe-design algorithm, library-free sequencing and an analysis pipeline provides a flexible and scalable method for quantifying cytosine methylation.
-
Article
The metabolome of induced pluripotent stem cells reveals metabolic changes occurring in somatic cell reprogramming
Metabolism is vital to every aspect of cell function, yet the metabolome of induced pluripotent stem cells (iPSCs) remains largely unexplored. Here we report, using an untargeted metabolomics approach, that hu...
-
Article
Increased methylation variation in epigenetic domains across cancer types
Andrew Feinberg and colleagues report whole-genome bisulfite sequencing of three colon cancers and matched normal tissue and two adenomatous polyps. The authors identify large blocks of relative hypomethylatio...
-
Article
Genome-wide map** of the sixth base
Map** of 5-hydroxylmethylcytosine in mammalian genomes has unveiled its unique role in the epigenetic regulation of gene expression.
-
Article
Recapitulation of premature ageing with iPSCs from Hutchinson–Gilford progeria syndrome
The premature ageing disorder Hutchinson–Gilford progeria syndrome (HGPS) is a rare genetic condition characterized by a rapid onset of signs associated with normal ageing, such as atherosclerosis and the dege...
