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Protocol
Multiplexing Autoradiography
Autoradiography, the direct imaging of radioactive distribution in tissue sections, is a powerful technique that has several key advantages for the validation of PET radiotracers. Using autoradiography, we can...
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Article
Open AccessPositron emission tomography imaging of the sodium iodide symporter senses real-time energy stress in vivo
Tissue environment is critical in determining tumour metabolic vulnerability. However, in vivo drug testing is slow and waiting for tumour growth delay may not be the most appropriate endpoint for metabolic tr...
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Article
Open AccessRadiosynthesis and Analysis of (S)-4-(3-[18F]Fluoropropyl)-L-Glutamic Acid
(S)-4-(3-[18F]Fluoropropyl)-L-glutamic acid ([18F]FSPG) is an L-glutamate derivative used as a PET biomarker to assess intracellular redox status in vivo through targeting of the cystine/glutamate antiporter prot...
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Article
Open AccessHepatic glutamine synthetase controls N5-methylglutamine in homeostasis and cancer
Glutamine synthetase (GS) activity is conserved from prokaryotes to humans, where the ATP-dependent production of glutamine from glutamate and ammonia is essential for neurotransmission and ammonia detoxificat...
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Article
The amino acid transporter SLC7A5 is required for efficient growth of KRAS-mutant colorectal cancer
Oncogenic KRAS mutations and inactivation of the APC tumor suppressor co-occur in colorectal cancer (CRC). Despite efforts to target mutant KRAS directly, most therapeutic approaches focus on downstream pathways,...
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Protocol
Imaging Cancer Metabolism with Positron Emission Tomography (PET)
Positron emission tomography (PET) enables the noninvasive spatiotemporal analysis of cancer metabolism in vivo. Both natural and nonnatural PET tracers have been developed to assess metabolic pathways during ...
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Article
Open AccessIdentifying active vascular microcalcification by 18F-sodium fluoride positron emission tomography
Vascular calcification is a complex biological process that is a hallmark of atherosclerosis. While macrocalcification confers plaque stability, microcalcification is a key feature of high-risk atheroma and is...
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Article
Disruption of mouse Slx4, a regulator of structure-specific nucleases, phenocopies Fanconi anemia
Ketan Patel and colleagues report a new mouse model of Fanconi anemia. The authors show that mice deficient in Btbd12, the mouse ortholog of SLX4 (a new Fanconi anemia gene), phenocopy the human disease.
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Article
Lasing from CdSe/ZnS Quantum Rods in a Cylindrical Microcavity
Lasing from CdSe/ZnS quantum rods and quantum dots both in solution and in a film is studied by utilizing a high Q cylindrical microcavity, showing Whispering Gallery Mode (WGM) lasing. CdSe/ZnS quantum rods, ...
