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Open AccessAuthor Correction: Critical shifts in lipid metabolism promote megakaryocyte differentiation and proplatelet formation
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Open AccessCritical shifts in lipid metabolism promote megakaryocyte differentiation and proplatelet formation
During megakaryopoiesis, megakaryocytes (MKs) undergo cellular morphological changes with strong modification of membrane composition and lipid signaling. Here, we adopt a lipid-centric multiomics approach to ...
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Article
Open AccessDivalent nanobodies to platelet CLEC-2 can serve as agonists or antagonists
CLEC-2 is a target for a new class of antiplatelet agent. Clustering of CLEC-2 leads to phosphorylation of a cytosolic YxxL and binding of the tandem SH2 domains in Syk, crosslinking two receptors. We have rai...
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Article
Open AccessPlatelet glycoprotein V spatio-temporally controls fibrin formation
The activation of platelets and coagulation at vascular injury sites is crucial for hemostasis but can promote thrombosis and inflammation in vascular pathologies. Here, we delineate an unexpected spatio-tempo...
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Author Correction: Platelet GPIbα is a mediator and potential interventional target for NASH and subsequent liver cancer
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Open AccessFoudroyant cerebral venous (sinus) thrombosis triggered through CLEC-2 and GPIIb/IIIa dependent platelet activation
Cerebral venous (sinus) thrombosis (CVT) is an unusual manifestation of venous thrombosis causing severe neurological impairment and seizures1,2. Molecular mechanisms underlying CVT, potentially involving patholo...
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Open AccessInfarct growth precedes cerebral thrombosis following experimental stroke in mice
Ischemic stroke is among the leading causes of disability and death worldwide. In acute ischemic stroke, successful recanalization of occluded vessels is the primary therapeutic aim, but even if it is achieved...
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Open AccessPlatelets and lymphocytes drive progressive penumbral tissue loss during middle cerebral artery occlusion in mice
In acute ischemic stroke, cessation of blood flow causes immediate tissue necrosis within the center of the ischemic brain region accompanied by functional failure in the surrounding brain tissue designated th...
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Article
Platelet GPIbα is a mediator and potential interventional target for NASH and subsequent liver cancer
Non-alcoholic fatty liver disease ranges from steatosis to non-alcoholic steatohepatitis (NASH), potentially progressing to cirrhosis and hepatocellular carcinoma (HCC). Here, w...
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Optical Clearing of Murine Bones to Study Megakaryocytes in Intact Bone Marrow Using Light-Sheet Fluorescence Microscopy
In mammals, the differentiation and maturation of megakaryocytes (MKs) occurs in the bone marrow (BM). The three-dimensional environment influences megakaryopoiesis and platelet release. Thus, imaging MKs with...
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Article
Open AccessThrombopoiesis is spatially regulated by the bone marrow vasculature
In mammals, megakaryocytes (MKs) in the bone marrow (BM) produce blood platelets, required for hemostasis and thrombosis. MKs originate from hematopoietic stem cells and are thought to migrate from an endostea...
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Open AccessA Cdc42/RhoA regulatory circuit downstream of glycoprotein Ib guides transendothelial platelet biogenesis
Blood platelets are produced by large bone marrow (BM) precursor cells, megakaryocytes (MKs), which extend cytoplasmic protrusions (proplatelets) into BM sinusoids. The molecular cues that control MK polarizat...
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Article
Open AccessPLD1 participates in BDNF-induced signalling in cortical neurons
The brain-derived neurotrophic factor BDNF plays a critical role in neuronal development and the induction of L-LTP at glutamatergic synapses in several brain regions. However, the cellular and molecular mecha...
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Correction: Corrigendum: Megakaryocyte-specific Profilin1-deficiency alters microtubule stability and causes a Wiskott–Aldrich syndrome-like platelet defect
Nature Communications 5: Article number: 4746 (2014); Published: 4 September 2014; Updated: 6 March 2015 In the original version of this Article, the middle initial of the author Katrin G. Heinze was omitted f...
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Megakaryocyte-specific Profilin1-deficiency alters microtubule stability and causes a Wiskott–Aldrich syndrome-like platelet defect
Wiskott–Aldrich syndrome (WAS) is caused by mutations in the WAS gene and is characterized by immunodeficiency, eczema and microthrombocytopenia. The molecular link between WAS mutations and microthrombocytopenia...
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Open AccessExcitotoxicity increases the release of 24S-hydroxycholesterol via CYP46A1 activation
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Endothelial TWIK-related potassium channel-1 (TREK1) regulates immune-cell trafficking into the CNS
Blood brain integrity is compromised in autoimmune disorders of the central nervous system (CNS0. Here Stefan Bittner et al. report that expression of TWIK-related potassium channel-1 (TREK1) is decreased in indi...
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Article
Platelet receptor signaling in thrombus formation
Platelet activation and subsequent thrombus formation at sites of vascular injury is crucial for normal hemostasis, but it can also cause myocardial infarction and stroke. The initial capture of flowing platel...
