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Article
Open AccessCompetition between inside-out unfolding and pathogenic aggregation in an amyloid-forming β-propeller
Studies of folded-to-misfolded transitions using model protein systems reveal a range of unfolding needed for exposure of amyloid-prone regions for subsequent fibrillization. Here, we probe the relationship be...
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Chapter and Conference Paper
An Optimization Strategy for Efficient Facial Landmark Detection Based on Improved Pixel-in-Pixel Net Model
Efficient facial landmark detection has been applied to various fields, such as driverless driving, facial beautification technology, facial expression analysis, etc. However, in specific practical tasks, ther...
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Article
Molecular mechanocytometry using tension-activated cell tagging
Flow cytometry is used routinely to measure single-cell gene expression by staining cells with fluorescent antibodies and nucleic acids. Here, we present tension-activated cell tagging (TaCT) to label cells fl...
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Article
Detection of cellular traction forces via the force-triggered Cas12a-mediated catalytic cleavage of a fluorogenic reporter strand
Molecular forces generated by cell receptors are infrequent and transient, and hence difficult to detect. Here we report an assay that leverages the CRISPR-associated protein 12a (Cas12a) to amplify the detect...
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Article
Open AccessA wild boar cathelicidin peptide derivative inhibits severe acute respiratory syndrome coronavirus-2 and its drifted variants
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) poses a clear threat to humanity. It has infected over 200 million and killed 4 million people worldwide, and infections continue with no end in...
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Article
Open AccessVaccine-induced protection against SARS-CoV-2 requires IFN-γ-driven cellular immune response
The overall success of worldwide mass vaccination in limiting the negative effect of the COVID-19 pandemics is inevitable, however, recent SARS-CoV-2 variants of concern, especially Omicron and its sub-lineage...
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Article
Open AccessPlatelet glycoprotein V spatio-temporally controls fibrin formation
The activation of platelets and coagulation at vascular injury sites is crucial for hemostasis but can promote thrombosis and inflammation in vascular pathologies. Here, we delineate an unexpected spatio-tempo...
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Chapter and Conference Paper
Exchange Rate Forecasting with Twitter Sentiment Analysis Technology
This paper attempts to introduce sentiment analysis technology into the task of exchange rate prediction, and studies the impact of sentiment factors on short-term fluctuation of exchange rate based on the the...
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Chapter and Conference Paper
AdCSE: An Adversarial Method for Contrastive Learning of Sentence Embeddings
Due to the impressive results on semantic textual similarity (STS) tasks, unsupervised sentence embedding methods based on contrastive learning have attracted much attention from researchers. Most of these app...
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Article
Open AccessAuthor Correction: Turn-key map** of cell receptor force orientation and magnitude using a commercial structured illumination microscope
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Article
Open AccessTurn-key map** of cell receptor force orientation and magnitude using a commercial structured illumination microscope
Many cellular processes, including cell division, development, and cell migration require spatially and temporally coordinated forces transduced by cell-surface receptors. Nucleic acid-based molecular tension ...
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Article
Open AccessIFN-γ-dependent NK cell activation is essential to metastasis suppression by engineered Salmonella
Metastasis accounts for 90% of cancer-related deaths and, currently, there are no effective clinical therapies to block the metastatic cascade. A need to develop novel therapies specifically targeting fundamen...
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Article
Open AccessActivation of von Willebrand factor via mechanical unfolding of its discontinuous autoinhibitory module
Von Willebrand factor (VWF) activates in response to shear flow to initiate hemostasis, while aberrant activation could lead to thrombosis. Above a critical shear force, the A1 domain of VWF becomes activated ...
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Chapter and Conference Paper
HMNet: Hybrid Matching Network for Few-Shot Link Prediction
Knowledge graphs (KGs) are widely used in many real-world applications, such as information retrieval, question answering system, and personal recommendation. However, most KGs are suffering from the incomplet...
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Article
Live-cell super-resolved PAINT imaging of piconewton cellular traction forces
Despite the vital role of mechanical forces in biology, it still remains a challenge to image cellular force with sub-100-nm resolution. Here, we present tension points accumulation for imaging in nanoscale to...
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Article
Open AccessNovel antibodies against GPIbα inhibit pulmonary metastasis by affecting vWF-GPIbα interaction
Platelet glycoprotein Ibα (GPIbα) extracellular domain, which is part of the receptor complex GPIb-IX-V, plays an important role in tumor metastasis. However, the mechanism through which GPIbα participates in ...
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Article
Genetic analysis of heterogeneous sub-clones in recombinant Chinese hamster ovary cells
Chinese hamster ovary (CHO) cells have been widely used for production of recombinant proteins and therapeutic antibodies. However, owing to the instability and heterogeneity of CHO cells, the development of s...
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Article
Open AccessPlatelet clearance via shear-induced unfolding of a membrane mechanoreceptor
Mechanisms by which blood cells sense shear stress are poorly characterized. In platelets, glycoprotein (GP)Ib–IX receptor complex has been long suggested to be a shear sensor and receptor. Recently, a relativ...
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Article
Open AccessStructural basis for the specific inhibition of glycoprotein Ibα shedding by an inhibitory antibody
Ectodomain shedding of glycoprotein (GP) Ibα is thought to mediate the clearance of activated, aged or damaged platelets. A monoclonal antibody, 5G6, has been developed recently to specifically bind to the GPI...
