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Open AccessNot1 and Not4 inversely determine mRNA solubility that sets the dynamics of co-translational events
The Ccr4-Not complex is mostly known as the major eukaryotic deadenylase. However, several studies have uncovered roles of the complex, in particular of the Not subunits, unrelated to deadenylation and relevan...
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Article
Open AccessGPR68 limits the severity of chemical-induced oral epithelial dysplasia
Head and neck cancer is the sixth most common malignancy, and there is an urgent need to identify physiological processes contributing to tumorigenesis. Extracellular acidification caused by aerobic glycolysis...
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Empyema Thoracis
Empyema thoracis, from the Greek, is defined simply as “pus in the pleural cavity.” Causes are multifactorial, although the majority results from bacterial pneumonia with subsequent parapneumonic effusion. Wit...
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Open AccessPitfalls in using phenanthroline to study the causal relationship between promoter nucleosome acetylation and transcription
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Opposing chromatin remodelers control transcription initiation frequency and start site selection
Precise nucleosome organization at eukaryotic promoters is thought to be generated by multiple chromatin remodeler (CR) enzymes and to affect transcription initiation. Using an integrated analysis of chromatin...
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Article
Open AccessRif1 S-acylation mediates DNA double-strand break repair at the inner nuclear membrane
Rif1 is involved in telomere homeostasis, DNA replication timing, and DNA double-strand break (DSB) repair pathway choice from yeast to human. The molecular mechanisms that enable Rif1 to fulfill its diverse r...
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ChECing out Rif1 action in freely cycling cells
In buddying yeast, like all eukaryotes examined so far, DNA replication is under temporal control, such that some origins fire early and some late during S phase. This replication timing program is established...
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Rif1 maintains telomeres and mediates DNA repair by encasing DNA ends
Structure determination and functional analyses of budding yeast Rif1 reveal a novel, hooked N-terminal DNA-binding domain required for telomere maintenance and checkpoint control and show that Rif1's role in ...
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Article
Open AccessA potent broad-spectrum protective human monoclonal antibody crosslinking two haemagglutinin monomers of influenza A virus
Effective annual influenza vaccination requires frequent changes in vaccine composition due to both antigenic shift for different subtype hemagglutinins (HAs) and antigenic drift in a particular HA. Here we pr...
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Regulation of telomere addition at DNA double-strand breaks
Telomeres constitute the ends of linear eukaryotic chromosomes. Due to the conventional mode of DNA replication, telomeric DNA erodes at each cell division. To counteract this, a specialized reverse transcript...
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Open AccessGlobal control of DNA replication timing by the budding yeast telomere protein Rif1
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Anticheckpoint pathways at telomeres in yeast
How yeast telomeres avoid triggering DNA damage checkpoints is studied by creating de novo telomere ends with different amounts of telomeric repeats. Rif1 and Rif2 protect short telomere tracts through different ...
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A conserved motif within RAP1 has diversified roles in telomere protection and regulation in different organisms
Telomere protein RAP1 is found in organisms from yeast to mammals, but has different functions. Now the crystal structures of the RAP1 C-terminus (RCT) with its different interacting partners, together with fu...
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Human Subject Protection and Special Population Issues
Understanding the principles and processes for protecting human subject (HS) safety is paramount in successfully carrying out clinical research. This chapter outlines the obligations that you have to HS protec...
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Article
Growth-regulated recruitment of the essential yeast ribosomal protein gene activator Ifh1
Regulation of ribosome biogenesis is central to the control of cell growth1. In rapidly growing yeast cells, ribosomal protein (RP) genes account for approximately one-half of all polymerase II transcription-init...
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Chromosomal landscape of nucleosome-dependent gene expression and silencing in yeast
Eukaryotic genomes are packaged into nucleosomes, which are thought to repress gene expression generally1,2,3. Repression is particularly evident at yeast telomeres, where genes within the telomeric heterochromat...
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Different means to common ends
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The means to bind the ends
The structure of a protein–telomere DNA complex from the yeast Saccharamyces cerevisiae suggests a highly conserved mode of telomere recognition.
