19 Result(s)
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Frank C. P. Holstege
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Article
Open AccessSystematic discovery of gene fusions in pediatric cancer by integrating RNA-seq and WGS
Gene fusions are important cancer drivers in pediatric cancer and their accurate detection is essential for diagnosis and treatment. Clinical decision-making requires high confidence and precision of detection...
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Open AccessSingle-cell transcriptomics reveals immune suppression and cell states predictive of patient outcomes in rhabdomyosarcoma
Paediatric rhabdomyosarcoma (RMS) is a soft tissue malignancy of mesenchymal origin that is thought to arise as a consequence of derailed myogenic differentiation. Despite intensive treatment regimens, the pro...
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Open AccessPPARγ lipodystrophy mutants reveal intermolecular interactions required for enhancer activation
Peroxisome proliferator-activated receptor γ (PPARγ) is the master regulator of adipocyte differentiation, and mutations that interfere with its function cause lipodystrophy. PPARγ is a highly modular protein,...
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Open AccessIdentification and characterization of relapse-initiating cells in MLL-rearranged infant ALL by single-cell transcriptomics
Infants with MLL-rearranged infant acute lymphoblastic leukemia (MLL-r iALL) undergo intense therapy to counter a highly aggressive malignancy with survival rates of only 30–40%. The majority of patients initiall...
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Open AccessA systematic analysis of genetic interactions and their underlying biology in childhood cancer
Childhood cancer is a major cause of child death in developed countries. Genetic interactions between mutated genes play an important role in cancer development. They can be detected by searching for pairs of ...
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Open AccessAn organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity
Kidney tumours are among the most common solid tumours in children, comprising distinct subtypes differing in many aspects, including cell-of-origin, genetics, and pathology. Pre-clinical cell models capturing...
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Open AccessAn endometrial gene expression signature accurately predicts recurrent implantation failure after IVF
The primary limiting factor for effective IVF treatment is successful embryo implantation. Recurrent implantation failure (RIF) is a condition whereby couples fail to achieve pregnancy despite consecutive embr...
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Open AccessA high-resolution gene expression atlas of epistasis between gene-specific transcription factors exposes potential mechanisms for genetic interactions
Genetic interactions, or non-additive effects between genes, play a crucial role in many cellular processes and disease. Which mechanisms underlie these genetic interactions has hardly been characterized. Unde...
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Open AccessUterine Selection of Human Embryos at Implantation
Human embryos frequently harbor large-scale complex chromosomal errors that impede normal development. Affected embryos may fail to implant although many first breach the endometrial epithelium and embed in th...
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Early adipogenesis is regulated through USP7-mediated deubiquitination of the histone acetyltransferase TIP60
Transcriptional coregulators, including the acetyltransferase Tip60, have a key role in complex cellular processes such as differentiation. Whereas post-translational modifications have emerged as an important...
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Article
A central role for TFIID in the pluripotent transcription circuitry
High levels of TFIID, a basal transcription factor, are found to be essential to induce and maintain the transcriptional program of pluripotent cells.
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Distinct promoter dynamics of the basal transcription factor TBP across the yeast genome
Transcription initiation involves recruitment of key factors to promoters. Yeast TATA-binding protein (TBP) turnover is now examined genome-wide and genes transcribed by the three RNA polymerases found to have...
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Redox-sensitive cysteines bridge p300/CBP-mediated acetylation and FoxO4 activity
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An expression profile for diagnosis of lymph node metastases from primary head and neck squamous cell carcinomas
Metastasis is the process by which cancers spread to distinct sites in the body. It is the principal cause of death in individuals suffering from cancer. For some types of cancer, early detection of metastasis...
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Minimum information about a microarray experiment (MIAME)—toward standards for microarray data
Microarray analysis has become a widely used tool for the generation of gene expression data on a genomic scale. Although many significant results have been derived from microarray studies, one limitation has ...
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Redundant roles for the TFIID and SAGA complexes in global transcription
The transcription factors TFIID and SAGA are multi-subunit complexes involved in transcription by RNA polymerase II1,2. TFIID and SAGA contain common TATA-binding protein (TBP)-associated factor (TAFII) subunits ...
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Functional redundancy of two transcriptional coactivator complexes
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Combinatorial negative regulation of glucose-responsive genes in yeast
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Chromosomal landscape of nucleosome-dependent gene expression and silencing in yeast
Eukaryotic genomes are packaged into nucleosomes, which are thought to repress gene expression generally1,2,3. Repression is particularly evident at yeast telomeres, where genes within the telomeric heterochromat...
