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Article
Langmuir–Blodgett nanotemplates for protein crystallography
Protein crystallization still presents a challenge for X-ray crystallography. This protocol describes the Langmuir–Blodgett nanotemplate method, in which 2D protein LB nanotemplates trigger formation of 3D pro...
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Chapter
Lichen Planus
Oral Lichen Planus (OLP), a common chronic inflammatory T-cell mediated oral mucocutaneous disease, is a complex oral disease and the management of a complex disorder requires a personalized treatment based on...
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Article
CHO Proteome Alterations Induced by Reverse Transformation
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Article
DNA bridging of yeast chromosomes VIII leads to near-reciprocal translocation and loss of heterozygosity with minor cellular defects
Loss of heterozygosity (LOH) of tumor suppressor genes in somatic cells is a major process leading to several types of cancer; however, its underlying molecular mechanism is still poorly understood. In the pre...
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Article
Objective assessment of scientific performances world-wide
In order to identify the indicators having world-wide standards for the assessment of scientific performances at the level of both individual and institutions normalized for disciplines, we have carried out a ...
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Article
Expression, purification and characterisation of a novel mutant of the human protein kinase CK2
The recombinant catalytic subunit of human protein kinase CK2 bas been mutagenised at the C-terminal region in an attempt to induce this tail to fold. We suppose in fact that this unstructured C-terminus just ...
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Book
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Chapter
Future Trends
Some time ago an alternative route to the dominant top-down approach in lithography and microelectronics was postulated by few authors (see Nicolini, 1989). This bottom-up approach aimed to the fabrication of ...
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Chapter
The New Frontier at the Crossing of Life and Physical Sciences
The present work is dedicated to the new frontiers of proteomics and crystallography based on nanoscience and nanotechnology (Nicolini, 1989; Nicolini 1996b; Rossof 2001; Nicolini et al, 2001a) with emphasis o...
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Chapter
State of the Art in Proteomics, Crystallography and Nanobiotechnology
With the development of genomics and proteomics, and their application in basic biological research and biotechnologies, there is an increasing need of functional and structural protein characterization. This ...
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Chapter
From Art to Science in Protein Crystallography
The encouraging results obtained with a model system, such as chicken egg white lysozyme that is summarized in the first part of this chapter, has opened new opportunities in the use of Langmuir-Blodgett prote...
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Chapter
Nanocrystallography
This chapter concerns the definition, the principles and the experimental layout of nanocrystallography which represents a significant advacement in protein crystallography. Nanocrystallography is indeed a new...
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Chapter
From Science to Technology in Proteomics
The recent advances in automated mass spectrometry, biomolecular arrays and industrial pharmaceutics constitute three clear examples of a transition from science to technology. In figure 5.1 are shown two exam...
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Article
Effects of polyamines on higher-order folding of in situ chromatin
Modifications of the higher-order chromatin structure induced by polyamines have been quantitatively investigated in situ through a non-invasive biophysical approach using Differential Scanning Calorimetry and...
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Article
X-ray small angle scattering study of chromatin as a function of fiber length
This work investigates the structure of native calf thymus chromatin as a function of fiber length and isolation procedures by using X-ray small angle scattering technique. Two methods of chromatin isolation h...
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Book Series
Electronics and Biotechnology Advanced (EL.B.A.) Forum Series
Volume 1 / 1995 to Volume 3 / 1998
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Book
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Chapter
Metalloprotein Engineering for New Materials, Drugs and Nanodevices
The purpose of this chapter is to provide an overview of present efforts on the engineering thin films of of various metalloproteins, likewise P450 and C cytochromes and Photosynthetic Reaction Center (RC), fr...
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Chapter
Single Electron and Quantum Phenomena in Ultra Small Particles
Principles, responsible for the single-electron phenomena, are considered. Different ways of the junction formation are discussed, emphasising that, based on the utilization of nanometer size granules. Model, ...
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Article
High order DNA structure as inferred by optical fluorimetry and scanning calorimetry
New quantitative insights on the native high order chromatin-DNA structure existing within interphase nuclei are obtained by monitoring the effects of two common well-characterized fixatives, glutaraldehyde an...
