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  1. No Access

    Chapter

    Functional genetics of Yarrowia lipolytica

    The yeast Yarrowia lipolytica has been used for industrial bioconversions since the late 1960’s, mainly by the food and chemical industries. The most important process is presently the production of citric acid f...

    Gerold Barth, Jean-Marie Beckerich… in Functional Genetics of Industrial Yeasts (2003)

  2. No Access

    Article

    Cloning the Yarrowia lipolytica homologue of the Saccharomyces cerevisiae SEC62 gene

    Yarrowia lipolytica SEC62 cDNA was cloned by functional complementation of a thermo-sensitive sec62 Saccharomyces cerevisiae mutant strain. The Y. lipolytica SEC62 promoter region was amplified by the inverse po...

    D. Swennen, Philippe Joyet, Claude Gaillardin in Current Genetics (1997)

  3. No Access

    Chapter

    Yarrowia lipolytica

    Interest in Candida lipolytica (Harrison) Diddens et Lodder 1942 initially arose from its rather uncommon physiological characteristics. Strains of this species were more often isolated from lipid- or protein-con...

    Gerold Barth, Claude Gaillardin in Nonconventional Yeasts in Biotechnology (1996)

  4. No Access

    Article

    Molecular cloning of Rab-related genes in the yeast Yarrowia lipolytica. Analysis of RYL1, an essential gene encoding a SEC4 homologue

    Small GTP-binding proteins of the Rab family are involved in the vesicular traffic inside eukaryotic cells. A gene library from the yeast Yarrowia lipolytica was screened with an oligonucleotide deduced from a hi...

    Brigitte Pertuiset, Jean-Marie Beckerich, Claude Gaillardin in Current Genetics (1995)

  5. No Access

    Article

    Multiple-copy integration in the yeast Yarrowia lipolytica

    Using an EcoRI-BglII fragment of the G unit of the rDNA of Y. lipolytica and a set of 11 deletions in the URA3 promoter, we have constructed several plasmids to test gene amplification in the rDNA. These plasmids...

    Marie-Thérèse Le Dall, Jean-Marc Nicaud, Claude Gaillardin in Current Genetics (1994)

  6. No Access

    Article

    LYC1 is the structural gene for lysine N-6-acetyl transferase in yeast

    In the yeastYarrowia lipolytica, theLYC1 locus controls the first step of the lysine degradation pathway which is catalyzed by lysine N-6-acetyl transferase (LAT). This gene was cloned by complementation of thely...

    Jean-Marie Beckerich, Micheline Lambert, Claude Gaillardin in Current Genetics (1994)

  7. No Access

    Article

    Chromosomal polymorphism of the yeast Yarrowia lipolytica and related species: electrophoretic karyoty** and hybridization with cloned genes

    Significant differences in electrophoretic karyoty** patterns were found among 27 strains of Y. lipolytica. Twenty-one of these strains were classified into four groups of similar karyotypes while six strains s...

    Elena Naumova, Gennadi Naumov, Philippe Fournier, Huu-Vang Nguyen… in Current Genetics (1993)

  8. No Access

    Article

    The yeast Yarrowia lipolytica has two, functional, signal recognition particle 7S RNA genes

    Cells containing a deletion of either the SCR1 or SCR2 genes, which code for the 7SL RNA component of the signal recognition particle (SRP) homologue, were found to be viable. Two independent approaches demonstra...

    Feng He, Debbie Yaver, Jean-Marie Beckerich, David Ogrydziak… in Current Genetics (1990)

  9. No Access

    Article

    Expression of invertase activity in Yarrowia lipolytica and its use as a selective marker

    Few selective markers are available for the transformation of the industrial yeast Yarrowia lipolytica, and those that are require the use of specialized hosts (e.g., auxotrophs, antibiotic sensitive). To enable ...

    Jean-Marc Nicaud, Emmanuelle Fabre, Claude Gaillardin in Current Genetics (1989)

  10. No Access

    Article

    Cloning of the LYS5 gene encoding saccharopine dehydrogenase from the yeast Yarrowia lipolytica by target integration

    A Yarrowia lipolytica yeast gene bank has been constructed in E. coli in an integrative plasmid vector containing the homologous LEU2 gene, and used to transform a leu2 lys5 yeast strain. The LYS5 gene encoding s...

    Jian-Wu Xuan, Philippe Fournier, Claude Gaillardin in Current Genetics (1988)

  11. No Access

    Article

    LEU2 directed expression of β-galactosidase activity and phleomycin resistance in Yarrowia lipolytica

    The nucleotide sequence of a 968 by DNA fragment spanning the promoter and the 5′ upstream sequence of the LEU2 coding sequence of the yeast Yarrowia lipolytica has been determined. A LEU2:lacZ gene fusion has be...

    Claude Gaillardin, Anne-Marie Ribet in Current Genetics (1987)

  12. No Access

    Article

    Replication and recombination of 2-µm DNA in Schizosaccharomyces pombe

    Any one of the inverted sequences present on the 2-µm DNA from Saccharomyces cerevisiae can promote replication of chimeric plasmids in Schizosaccharomyces pombe. When however a complete 2-µm molecule is present ...

    Claude Gaillardin, Philippe Fournier, Françoise Budar, Bernard Kudla… in Current Genetics (1983)