30 Result(s)
within
Christopher B. Little
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Article
Open AccessLoss of Grem1-lineage chondrogenic progenitor cells causes osteoarthritis
Osteoarthritis (OA) is characterised by an irreversible degeneration of articular cartilage. Here we show that the BMP-antagonist Gremlin 1 (Grem1) marks a bipotent chondrogenic and osteogenic progenitor cell pop...
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Open AccessIn Vitro Collagenase Degradation of Grafts Used Clinically for Anterior Cruciate Ligament Reconstruction: Human Tendon Data
Increasing anterior cruciate ligament (ACL) rupture rates are driving the need for new graft materials which undergo testing to characterize material properties and function. The in vitro collagenase assay is ...
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Open AccessMuscle spindles of the multifidus muscle undergo structural change after intervertebral disc degeneration
Proprioceptive deficits are common in low back pain. The multifidus muscle undergoes substantial structural change after back injury, but whether muscle spindles are affected is unclear. This study investigate...
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Open AccessDiurnal effects of polypharmacy with high drug burden index on physical activities over 23 h differ with age and sex
Aging, polypharmacy (concurrent use of ≥ 5 medications), and functional impairment are global healthcare challenges. However, knowledge of the age/sex-specific effects of polypharmacy is limited, particularly ...
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Open AccessThe relationship between synovial inflammation, structural pathology, and pain in post-traumatic osteoarthritis: differential effect of stem cell and hyaluronan treatment
Synovitis is implicated in the severity and progression of pain and structural pathology of osteoarthritis (OA). Increases in inflammatory or immune cell subpopulations including macrophages and lymphocytes ha...
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Open AccessDevelo** strategic priorities in osteoarthritis research: Proceedings and recommendations arising from the 2017 Australian Osteoarthritis Summit
There is a pressing need to enhance osteoarthritis (OA) research to find ways of alleviating its enormous individual and societal impact due to the high prevalence, associated disability, and extensive costs.
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Article
Macrophage polarization contributes to local inflammation and structural change in the multifidus muscle after intervertebral disc injury
Intervertebral disk (IVD) lesion and its subsequent degeneration have a profound effect on the multifidus muscle. The subacute/early chronic phase of multifidus remodeling after IVD lesion has been proposed to...
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Article
Open AccessComprehensive Expression Analysis of microRNAs and mRNAs in Synovial Tissue from a Mouse Model of Early Post-Traumatic Osteoarthritis
To better understand the molecular processes involved in driving osteoarthritis disease progression we characterized expression profiles of microRNAs (miRNA) and mRNAs in synovial tissue from a post-traumatic ...
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Article
Open AccessOn the predictive utility of animal models of osteoarthritis
Animal models of osteoarthritis are extensively used for investigating disease pathways and for preclinical testing of novel therapies. Their predictive utility, however, has often been questioned, mainly beca...
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Open AccessEndothelial protein C receptor-associated invasiveness of rheumatoid synovial fibroblasts is likely driven by group V secretory phospholipase A2
Rheumatoid synovial fibroblasts (RASFs) mediate joint inflammation and destruction in rheumatoid arthritis (RA). Endothelial protein C receptor (EPCR) is a specific receptor for the natural anticoagulant activ...
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Article
The ovine newborn and human foetal intervertebral disc contain perlecan and aggrecan variably substituted with native 7D4 CS sulphation motif: spatiotemporal immunolocalisation and co-distribution with Notch-1 in the human foetal disc
Composite agarose (1.2 %) polyacrylamide (0.6 %) gel electrophoresis was used to separate discrete populations of native aggrecan and perlecan in newborn to 10 year old ovine intervertebral discs (IVDs). Semi-...
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Article
Comparative immunolocalisation of perlecan, heparan sulphate, fibroblast growth factor-18, and fibroblast growth factor receptor-3 and their prospective roles in chondrogenic and osteogenic development of the human foetal spine
A comparative immunolocalisation study of perlecan, HS, FGF-18 and FGFR-3 in the 12–20-week gestational age human foetal spine was undertaken to identify spatiotemporal associations between these components to...
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Article
Post-traumatic osteoarthritis: from mouse models to clinical trials
In this Review, Little and Hunter discuss the use of animal models in osteoarthritis (OA) research, focusing on their importance in understanding post-traumatic OA, the human form of the disease that the model...
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Open AccessA hexadecylamide derivative of hyaluronan (HYMOVIS®) has superior beneficial effects on human osteoarthritic chondrocytes and synoviocytes than unmodified hyaluronan
Intra-articular hyaluronan (HA) injection provides symptomatic benefit in the treatment of osteoarthritis (OA). Previously we found superior beneficial effects in a large animal OA model of a hexadecylamide de...
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Article
Chondroitin sulphate and heparan sulphate sulphation motifs and their proteoglycans are involved in articular cartilage formation during human foetal knee joint development
Novel sulphation motifs within the glycosaminoglycan chain structure of chondroitin sulphate (CS) containing proteoglycans (PGs) are associated with sites of growth, differentiation and repair in many biologic...
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Article
The interaction of canonical bone morphogenetic protein- and Wnt-signaling pathways may play an important role in regulating cartilage degradation in osteoarthritis
Bone morphogenetic proteins (BMPs) and Wnts are important signaling protein families with key roles in embryologic, patterning, development, and tissue remodeling in growth. BMP and Wnt-β-catenin are highly ev...
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Article
Mutations in TRPV4 cause an inherited arthropathy of hands and feet
Shireen Lamandé and colleagues report mutations in TRPV4 in familial digital arthropathy-brachydactyly (FDAB), which is characterized by osteoarthropathy of the fingers and toes. TRPV4 is a cation channel, and fu...
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Article
Investigating ADAMTS-mediated aggrecanolysis in mouse cartilage
Proteolysis of the cartilage proteoglycan aggrecan is a feature of arthritis. We present a method for analyzing aggrecanolysis in in vitro cultures of 3-week-old mouse femoral head cartilage based on traditional ...
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Article
Open AccessS100A8 and S100A9 in experimental osteoarthritis
The objective was to evaluate the changes in S100A8 S100A9, and their complex (S100A8/S100A9) in cartilage during the onset of osteoarthritis (OA) as opposed to inflammatory arthritis.
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Neoepitope Antibodies Against MMP-Cleaved and Aggrecanase-Cleaved Aggrecan
Neoepitope antibodies recognize the newly created N or C terminus of protein degradation products but fail to recognize the same sequence of amino acids present in intact or undigested protein. Aggrecan neoepi...
