17 Result(s)
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Article
Widdrol-induced lipolysis is mediated by PKC and MEK/ERK in 3T3-L1 adipocytes
Obesity is a serious medical condition causing various diseases such as heart disease, type-2 diabetes, and cancer. Fat cells (adipocytes) play an important role in the generation of energy through hydrolysis ...
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Widdrol activates DNA damage checkpoint through the signaling Chk2–p53–Cdc25A–p21–MCM4 pathway in HT29 cells
Widdrol is an odorant compound isolated from Juniperus chinensis. We previously reported that widdrol induces Gap 1 (G1) phase cell cycle arrest and leads to apoptosis in human colon adenocarcinoma HT29 cells. It...
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Article
Changes in vascular endothelial growth factor (VEGF) induced by the Morris water maze task
The present study was undertaken to evaluate the effects on hippocampal vascular endothelial growth factor (VEGF) levels in rats when they experience hippocampal-dependent spatial learning via the Morris water...
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Article
A critical time window for the survival of neural progenitor cells by HDAC inhibitors in the hippocampus
Histone deacetylase inhibitors (HDACIs) that modulate gene expression by inhibiting HDAC enzymes may contribute to the survival of immature hippocampal neurons. However, it remains unknown how and when HDACIs ...
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Molecular cloning and characterization of the gene encoding a fibrinolytic enzyme from Bacillus subtilis Strain A1
A fibrinolytic metalloprotease gene from Bacillus subtilis has been cloned in Escheridria coliXL1-Blue and the bacterial expressed enzyme was purified. The nucleotide sequence of the cloned fibrinolytic enzyme ge...
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Cloning and characterization of an exoinulinase from Bacillus polymyxa
A gene encoding an exoinulinase (inu) from Bacillus polymyxa MGL21 was cloned and sequenced. It is composed of 1455 nucleotides, encoding a protein (485 amino acids) with a molecular mass of 55 522 Da. Inu was ex...
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Increased poly-β-hydroxybutyrate (PHB) chain length by the modulation of PHA synthase activity in recombinant Escherichia coli
A second promoter (P1) was inserted to the PHA (poly-β-hydroxyalkanoate) operon (pSP2) of Esherichia coli DH5α with an optimal E. coli ribosome binding site and a trc strong promoter (pSJS1) to obtain poly-β-hydr...
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Article
Expression of Bacillus macerans cyclodextrin glucanotransferase gene in Saccharomyces cerevisiae
Cyclodextrin glucanotransferase (CGTase) gene of Bacillus macerans was subcloned down-stream of yeast ADH1 promoter and expressed in Saccharomyces cerevisiae. Most of the CGTase expressed was in the extracellular...
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Article
Interferometeric sensing of β-galactosidase released by recombinant E. coli responding to an endocrine disruptor, tributyltin
A recombinant E. coli ACV1003 releasing β-galactosidase by a SOS regulon system when it is exposed to a DNA-damaging compound, has been used to detect endocrine disruptors such as tributyltin (TBT) and triphenylt...
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Article
Two-phase extraction culture of Botryococcus braunii producing long-chain unsaturated hydrocarbons
Hydrocarbon recovery was 32% and the total production of hydrocarbon in a two-phase extraction culture of Botryococcus braunii was 675 mg l−1, which was 19% higher than that of single-phase culture. Addition of 6...
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Article
Production of fructosylxyloside by the new fructosyltransferase from Bacillus macerans EG-6
The new fructosyltransferase (FTase) from Bacillus maceransEG-6 showed a broad acceptor specificity, and resulted in the formation of fructosylxyloside (FX) with d-xylose being the most effective acceptor. The op...
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Article
Expression of Bacillus sp. β-xylosidase gene (xylB) in Saccharomyces cerevisiae
β-Xylosidase gene (xylB) from Bacillus sp. was amplified and inserted between GAL10 promoter and GAL7 terminator. For the secretory production of xylB in Saccharomyces cerevisiae, in-frame fusion of the exoinulin...
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Selective production of GF4-fructooligosaccharide from sucrose by a new transfructosylating enzyme
A new fructosyltransferase produced by a new isolate of Bacillus macerans EG-6 showed mainly hydrolyzing activity at 10 g sucrose/l, while both hydrolyzing and transfructosylating activity were observed at 100...
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Article
Enhanced Microbial Removal of H2S Using Chlorobium in an Optical-Fiber Bioreactor
The removal rate of H2S in a scratched optical fiber bioreactor using Chlorobium thiosulfatophilum was 0.87 µmol H2S oxidized·l/min·mg protein, which was 6.7 times that in an external illuminating reactor. Availa...
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Article
Expression and secretion of Clostridium thermocellum endoglucanase A gene (celA) in different Saccharomyces cerevisiae strains
To develop effective and powerful probiotics, Saccharomyces cerevisiae strains producing cellulolytic enzymes were genetically engineered. Two plasmids were constructed in which the carboxymethyl cellulase (CM...
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Article
Comparison of sugar compositions between inulo- and fructo-oligosaccharides produced by different enzyme forms
The sugar composition of inulo-oligosaccharides produced from inulin consisted of mainly inulobiose and inulotriose in almost equal amounts with a bacterial endoinulinase either as a soluble enzyme or as intac...
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Bioconversion of hydrogen sulfide by free and immobilized cells ofChlorobium thiosulfatophilum
The transformation of hydrogen sulfide into elementary sulfur and sulfate was investigated in a photo-bioreactor using autotropic bacteriaChlorobium thiosulfatophilum. The accumulations of sulfur and sulfate in t...
