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Article
CRET-based immunoassay on magnetic beads for selective and sensitive detection of Nanog antigen as a key cancer stem cell marker
A method is presented for chemiluminescence resonance energy transfer (CRET) using APTES-Fe3O4 as a highly efficient energy acceptor with strong magnetic effectiveness over extended distances, while an Au@BSA-lum...
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Article
An insight into the potentials of carbon dots for in vitro live-cell imaging: recent progress, challenges, and prospects
Carbon dots (CDs) are a strong alternative to conventional fluorescent probes for cell imaging due to their brightness, photostability, tunable fluorescence emission, low toxicity, inexpensive preparation, and...
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Article
A Novel Strategy for Trinitrotoluene Detection Using Functionalized Gold Nanoparticles
Gold nanoparticles (AuNPs) with unique optical properties, such as easy operation and visualized assay, have a great ability to detect different types of analytes. In the present study, a novel AuNPs based aptase...
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Article
Open AccessEvaluation of Rationally Designed Label-free Stem-loop DNA Probe Opening in the Presence of miR-21 by Circular Dichroism and Fluorescence Techniques
The characteristic features of stem-loop structured probes make them robust tools to detect targets with high sensitivity and selectivity. The basis of the hairpin based sensors operation is a conformational c...
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Article
Design and Fabrication of a Silver Nanocluster-Based Aptasensor for Lysozyme Detection
Lysozyme is a universal part of the innate immune system and is present in various bodily fluids. It is also vastly studied as a biomarker for a variety of diseases and is used in the food industry as a preser...
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Article
Open AccessAssembly of Gold Nanorods on HSA Amyloid Fibrils to Develop a Conductive Nanoscaffold for Potential Biomedical and Biosensing Applications
Today, Gold Nanorods have promised variety of applications in conjugation with biomolecules of interest. Discovery of functional amyloids has also been highlighted with possible use in designing high performan...
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Article
Critical Role of a Loop at C-Terminal Domain on the Conformational Stability and Catalytic Efficiency of Chondroitinase ABC I
We used a combination of protein engineering and spectroscopic methods to investigate the effect of a long length loop on the conformational stability and activity of chondroitinase ABC I. This study involves ...
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Article
Plasmonic Circular Dichroism Study of DNA–Gold Nanoparticles Bioconjugates
Plasmonic circular dichroism (CD) responses of hybrid nanostructures containing noble metal nanoparticles and chiral molecules have received increasing interest with various applications in nanophotonics. Chir...
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Article
Extra EF Hand Unit (DX) Mediated Stabilization and Calcium Independency of α-Amylase
It is the common feature of α-amylases that calcium ion is required for their structural integrity and thermal stability. All amylases have at least one Ca2+ per molecule; therefore amino acids involved in calciu...
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Article
Artemin as an Efficient Molecular Chaperone
Artemin is an abundant thermostable protein in Artemia encysted embryos under stress. It is considered as a stress protein, as its highly regulated expression is associated with stress resistance in this crustace...
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Article
Acid-Induced Formation of Molten Globule States in the Wild Type Escherichia coli 5-Enolpyruvylshikimate 3-Phosphate Synthase and its Three Mutated Forms: G96A, A183T and G96A/A183T
Recent advances in protein chemistry have led to progress in the understanding of protein folding and properties of possible intermediates during the folding of proteins. The molten globule (MG) state, a major...
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Article
Characterization of Acid-Induced Partially Folded Conformation Resembling a Molten Globule State of Polygalacturonase from a Filamentous Fungus Tetracoccosporium sp.
Acid-induced unfolding of a Tetracoccosporium sp. polygalacturonase enzyme (PG) was studied by a comprehensive series of biophysical and biochemical techniques. At pH 1.0, PG acquires partially folded state, whic...
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Article
Relationship between stability and bioluminescence color of firefly luciferase
Firefly luciferase catalyzes the oxidation of luciferin in the presence of ATP, Mg2+ and molecular oxygen. The bioluminescence color of firefly luciferases is identified by the luciferase structure and assay cond...
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Article
Open AccessBinding of long-chain α-neurotoxin would stabilize the resting state of nAChR: A comparative study with α-conotoxin
The details of interaction in a complex between potent antagonists such as long chain α-neurotoxins and α-conotoxins with nicotinic acetylcholine receptor (nAChR), and conformational changes induced by these a...
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Article
PCR-based Gene Synthesis, Molecular Cloning, High Level Expression, Purification, and Characterization of Novel Antimicrobial Peptide, Brevinin-2R, in Escherichia Coli
Brevinin-2R, a member of a new family of antimicrobial peptides isolated from the skin of Rana ridibunda, displays antimicrobial activity against bacteria and fungi. In this study, we have used an assembly PCR me...
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Article
A stopped-flow fluorescence study of the native and modified lysozyme
The protein folding kinetics of hen egg white lysozyme (HEWL) was studied using experimental and bioinformatics tools. The structure of the transition state in the unfolding pathway of lysozyme was determined ...
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Article
Open AccessBinding site locations and interaction of the isolated middle loop of snake neurotoxin I with muscle type nicotinic acetylcholine receptor: molecular dynamics and docking simulation study
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Article
Purification, characterization, and structural investigation of a new moderately thermophilic and partially calcium-independent extracellular α-amylase from Bacillus sp. TM1
A new α-amylase was extracted from a recently found strain of Bacillus sp. and purified by ion-exchange chromatography. Sodium dodecyl sulfate polyacrylamide gel electrophoresis showed a single band for the purif...
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Article
Interaction of an intermediate structure of Bacillus subtilis α-amylase with alkyl-substituted sepharose 4B
An intermediate from of α-amylase from Bacillus subtilis was prepared following a previously reported procedure. Stabilization of this protein structure by various additives and its interaction with alkyl-substit...
