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Protocol
Protease Substrate Profiling by N-Terminal COFRADIC
Detection of (neo-)N-terminal peptides is essential for identifying protease . We here present an update of a well-established and efficient selection method for enriching N-terminal peptides out of peptide m...
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Protocol
In-Gel N-Acetylation for the Quantification of the Degree of Protein In Vivo N-Terminal Acetylation
Maturation of protein N-termini occurs in all kingdoms of life, with major protein modifications being proteolytic processing (e.g., removal of initiator methionines) and N-terminal acetylation. The functional...