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Article
Open AccessN-terminal cysteine acetylation and oxidation patterns may define protein stability
Oxygen homeostasis is maintained in plants and animals by O2-sensing enzymes initiating adaptive responses to low O2 (hypoxia). Recently, the O2-sensitive enzyme ADO was shown to initiate degradation of target pr...
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Article
Protein N-terminal acetylation is entering the degradation end game
Despite being one of the most common protein modifications in eukaryotes, the cellular effect of N-terminal acetylation remains cryptic. Four decades ago, the nature of the N terminus was linked to protein sta...
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Article
Open AccessBiallelic NAA60 variants with impaired N-terminal acetylation capacity cause autosomal recessive primary familial brain calcifications
Primary familial brain calcification (PFBC) is characterized by calcium deposition in the brain, causing progressive movement disorders, psychiatric symptoms, and cognitive decline. PFBC is a heterogeneous dis...
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Article
Open AccessN-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity
Most eukaryotic proteins are N-terminally acetylated, but the functional impact on a global scale has remained obscure. Using genome-wide CRISPR knockout screens in human cells, we reveal a strong genetic depe...
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Article
Open AccessLoss of N-terminal acetyltransferase A activity induces thermally unstable ribosomal proteins and increases their turnover in Saccharomyces cerevisiae
Protein N-terminal (Nt) acetylation is one of the most abundant modifications in eukaryotes, covering ~50-80 % of the proteome, depending on species. Cells with defective Nt-acetylation display a wide array of...
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Article
Open AccessBiochemical analysis of novel NAA10 variants suggests distinct pathogenic mechanisms involving impaired protein N-terminal acetylation
NAA10 is the catalytic subunit of the N-terminal acetyltransferase complex, NatA, which is responsible for N-terminal acetylation of nearly half the human proteome. Since 2011, at least 21 different NAA10 missens...
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Article
Open AccessX-chromosome variants are associated with aldosterone producing adenomas
Aldosterone-producing adenomas (APAs) are a major cause of primary aldosteronism (PA) and are characterized by constitutively producing aldosterone, which leads to hypertension. Several mutations have been ide...
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Article
Open AccessSevere syndromic ID and skewed X-inactivation in a girl with NAA10 dysfunction and a novel heterozygous de novo NAA10 p.(His16Pro) variant - a case report
NAA10 is the catalytic subunit of the major N-terminal acetyltransferase complex NatA which acetylates almost half the human proteome. Over the past decade, many NAA10 missense variants have been reported as c...
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Article
Open AccessMolecular basis for N-terminal acetylation by human NatE and its modulation by HYPK
The human N-terminal acetyltransferase E (NatE) contains NAA10 and NAA50 catalytic, and NAA15 auxiliary subunits and associates with HYPK, a protein with intrinsic NAA10 inhibitory activity. NatE co-translatio...
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Article
Open AccessA novel NAA10 p.(R83H) variant with impaired acetyltransferase activity identified in two boys with ID and microcephaly
N-terminal acetylation is a common protein modification in human cells and is catalysed by N-terminal acetyltransferases (NATs), mostly cotranslationally. The NAA10-NAA15 (NatA) protein complex is the major NA...
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Article
Open AccessSpotlight on protein N-terminal acetylation
N-terminal acetylation (Nt-acetylation) is a widespread protein modification among eukaryotes and prokaryotes alike. By appending an acetyl group to the N-terminal amino group, the charge, hydrophobicity, and ...
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Article
Open AccessInvestigating the functionality of a ribosome-binding mutant of NAA15 using Saccharomyces cerevisiae
N-terminal acetylation is a common protein modification that occurs preferentially co-translationally as the substrate N-terminus is emerging from the ribosome. The major N-terminal acetyltransferase complex A...
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Article
Open AccessNAA10 dysfunction with normal NatA-complex activity in a girl with non-syndromic ID and a de novo NAA10 p.(V111G) variant – a case report
The NAA10-NAA15 (NatA) protein complex is an N-terminal acetyltransferase responsible for acetylating ~ 40% of eukaryotic proteins. In recent years, NAA10 variants have been found in patients with an X-linked dev...
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Protocol
[14C]-Acetyl-Coenzyme A-Based In Vitro N-Terminal Acetylation Assay
N-terminal acetylation is one of the most abundant co- and posttranslational protein modifications, conserved from prokaryotes to eukaryotes. The functional consequences of this modification are manifold, rang...
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Protocol
DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity
We here describe a quick and easy method to quantitatively measure in vitro acetylation activity of not only N-terminal acetyltransferase (NAT) enzymes, but acetyltransferases using acetyl-coenzyme A as an ace...
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Article
Open AccessNaa50/San-dependent N-terminal acetylation of Scc1 is potentially important for sister chromatid cohesion
The gene separation anxiety (san) encodes Naa50/San, a N-terminal acetyltransferase required for chromosome segregation during mitosis. Although highly conserved among higher eukaryotes, the mitotic function of t...
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Article
Open AccessMicroscopy-based Saccharomyces cerevisiae complementation model reveals functional conservation and redundancy of N-terminal acetyltransferases
N-terminal acetylation is a highly abundant protein modification catalyzed by N-terminal acetyltransferases (NATs) NatA-NatG. The Saccharomyces cerevisiae protein Arl3 depends on interaction with Sys1 for its loc...
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Chapter
Educational Technology and Polycontextual Bridging
Digital media has a growing importance and it affects people’s communication habits and patterns and their attitudes towards school learning. We need greater understanding as to how learning takes place in var...
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Chapter
What Explains Pupils’ Perceived Motivational Conflict between Academic Work and Off-Task Behaviour in Technology-Rich Classrooms?
Self-discipline is more important than IQ in predicting academic performance of adolescents (Duckworth & Seligman, 2005) and is therefore important in classrooms where autonomous pupils are expected to manage ...
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Article
Open AccessNAA10 mutation causing a novel intellectual disability syndrome with Long QT due to N-terminal acetyltransferase impairment
We report two brothers from a non-consanguineous Irish family presenting with a novel syndrome characterised by intellectual disability, facial dysmorphism, scoliosis and long QT. Their mother has a milder phe...
