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    Protocol

    Protease Substrate Profiling by N-Terminal COFRADIC

    Detection of (neo-)N-terminal peptides is essential for identifying protease . We here present an update of a well-established and efficient selection method for enriching N-terminal peptides out of peptide m...

    An Staes, Petra Van Damme, Evy Timmerman, Bart Ruttens in Protein Terminal Profiling (2017)

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    Protocol

    In-Gel N-Acetylation for the Quantification of the Degree of Protein In Vivo N-Terminal Acetylation

    Maturation of protein N-termini occurs in all kingdoms of life, with major protein modifications being proteolytic processing (e.g., removal of initiator methionines) and N-terminal acetylation. The functional...

    Petra Van Damme, Thomas Arnesen, Bart Ruttens, Kris Gevaert in Protein Acetylation (2013)

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    Article

    Selecting protein N-terminal peptides by combined fractional diagonal chromatography

    In recent years, procedures for selecting the N-terminal peptides of proteins with analysis by mass spectrometry have been established to characterize protease-mediated cleavage and protein α-N-acetylation on a p...

    An Staes, Francis Impens, Petra Van Damme, Bart Ruttens, Marc Goethals in Nature Protocols (2011)