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Article
Chemotherapy resistance of mouse WAP-SVT/t breast cancer cells is mediated by osteopontin, inhibiting apoptosis downstream of caspase-3
Impairment of the complex regulatory network of cell death and survival is frequently the reason for therapy resistance of breast cancer cells and a major cause of tumor progression. We established two indepen...
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Article
The SV40 small t-antigen prevents mammary gland differentiation and induces breast cancer formation in transgenic mice; truncated large T-antigen molecules harboring the intact p53 and pRb binding region do not have this effect
We report here for the first time, that the SV40 small t-antigen inhibits mammary gland differentiation during mid-pregnancy and that about 10% of multiparous WAP-SVt transgenic animals develop breast tumors w...
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Article
SV40 T/t-antigen induces premature mammary gland involution by apoptosis and selects for p53 missense mutation in mammary tumors
We recently established transgenic animals (WAP-SV-T/t) carrying the early coding region of Simian Virus 40 (SV40) under the transcriptional control of the whey acidic milk protein promoter (WAP), which restri...
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Chapter
DNA Methylation, chromatin structure and the regulation of gene expression
It is still not clear how eukaryotic cells regulate gene expression during differentiation and in the differentiated state. There is increasing experimental evidence that this requires a wide spectrum of diffe...
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Chapter and Conference Paper
The Biological Activity of Early SV40 Antisense RNA and DNA Molecules
It is of basic and applied scientific interest to develop reliable techniques which allow the down regulation of single genes either in isolated cells or in cells of an intact organism. Such an approach should...
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Book
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Chapter
Gene Transfer into Culture Cells and its Application to Study Cell Transformation
Many aspects of the intracellular behaviour of macromolecules cannot be studied satisfactorily in cell free systems. During the last decades different techniques were developed to analyze the biological activi...
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Chapter
Microinjection Turns a Tissue Culture Cell into a Test Tube
The demand for techniques to investigate biologically important macro-molecules (DNA, RNA, proteins) within the living cell has increased continually with our growing competence to purify, analyze, and modify ...
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Chapter and Conference Paper
A Microinjection Technique Converting Living Cells into Test Tubes
Although techniques to micromanipulate and to inject individual cells had been devised already at the beginning of this century (Barber 1914), this approach did not come to prominence until the last two decade...
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Chapter
A Microinjection Technique Converting Living Cells into Test Tubes
Although techniques to micromanipulate and to inject individual cells had been devised already at the beginning of this century (Barber 1914), this approach did not come to prominence until the last two decade...
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Book
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Chapter
Biological Activity of Simian Virus 40 DNA Fragments and T-Antigen Tested by Microinjection into Tissue Culture Cells
The biological activity of macromolecules (DNA, RNA, protein) can be directly tested by microinjection into tissue culture cells (1, 2, 3). The transfer of the test material is performed with microglass capill...
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Article
Experimental evidence that polyoma-specific tumour antigen is a virus-coded protein
POLYOMA virus (PV) induces intranuclear tumour (T) antigen formation in permissive and semipermissive cells. In virus-infected cells, T antigen appears before the onset of viral DNA synthesis and is continuous...
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Article
The biological activity of different forms of Polyoma Virus DNA and viral DNA fragments
Mouse tissue culture cells were infected with different forms of Polyoma Virus (PV) DNA or virus DNA fragments by means of a microinjection technique and stained for PV-tumor (T) antigen and virus capsid (V) a...
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Chapter
Involvement of RNA in the Process of Puff Induction in Polytene Chromosomes
A variety of agents can induce puffs in the polytene chromosomes of dipteran salivary glands. Ecdysone and its analogues and temperature shocks are best suited for studies on the mechanism of puff induction be...
