Abstract
Although techniques to micromanipulate and to inject individual cells had been devised already at the beginning of this century (Barber 1914), this approach did not come to prominence until the last two decades when biologically important macromolecules had been purified and means were required to assay them within their natural environment: the living cell. We have developed a microinjection technique which employs glass microcapillaries for the transfer of biological materials into mammalian tissue culture cells. Since the technical aspects of the instrumentation and its handling have been described in detail recently (Graessmann et al. 1980; Celis et al. 1980), only a short summary of the essential steps will be given.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Barber MA (1914) Philippine J Sci 9: 307–362
Celis JE, Kaltoft K, Bravo R (1980) In: Celis JE, Graessmann A, Loyter A (eds) Transfer of cell constituents into eukaryotic cells. Plenum Press, New York London, p 1
Feramisco JR (1979) Proc Natl Acad Sci USA 76: 3967–3971
Graessmann A (1968) Mikrochirurgische Zellkern-transplantation in somatischen Säugetierzellen, Doct. Diss. Freie Univ. Berlin
Graessmann A, Graessmann M (1971) Hoppe Seylers Z Physiol Chem 352: 527–532
Graessmann M, Graessmann A (1976) Proc Natl Acad Sci USA 73: 366–370
Graessmann A, Graessmann M, Müller C (1979) Biochem Biophys Res Commun 88: 428–432
Graessmann A, Graessmann M, Müller C (1979a) Curr Top Microbiol Immunol 87: 1–21
Graessmann A, Graessmann M, Tjian R, Topp WC (1979b) J Virol 33: 1182–1191
Graessmann M, Graessmann A, Mueller C (1979) Cold Spring Harbor Symp Quant Biol 44: 605–610
Graessmann A, Graessmann M, Mueller C (1980) In: Grossmann L, Moldave K (eds) Methods in enzymology, vol 65. Academic Press, New York London Toronto Sydney San Francisco, p 816
Graessmann A, Wolf H, Bornkamm GW (1980a) Proc Natl Acad Sci USA 77: 433–436
Huez G, Bruck C, Portetelle D, Cleuter Y (1980) In: Celis JE, Graessmann A, Loyter A (eds) Transfer of cell constituents into eukaryotic cells. Plenum Press, New York London, p 55
Kreis TE, Winterhalter KH, Birchmeier W (1979) Proc Natl Acad Sci USA 76: 3814–3818
Mueller C, Graessmann A, Graessmann M (1978) Cell 15: 579–585
Tjian R, Fey G, Graessmann A (1978) Proc Natl Acad Sci USA 75: 1279–1283
Tooze J (ed) (1980) Molecular biology of tumor viruses, 2nd edn, part 2, DNA tumor viruses. Cold Spring Harbor Laboratory, New York, p 61
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1981 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Mueller, C., Graessmann, M., Graessmann, A. (1981). A Microinjection Technique Converting Living Cells into Test Tubes. In: Schweiger, H.G. (eds) International Cell Biology 1980–1981. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-39932-3_16
Download citation
DOI: https://doi.org/10.1007/978-3-662-39932-3_16
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-38972-0
Online ISBN: 978-3-662-39932-3
eBook Packages: Springer Book Archive