Abstract
The biological activity of macromolecules (DNA, RNA, protein) can be directly tested by microinjection into tissue culture cells (1, 2, 3). The transfer of the test material is performed with microglass capillaries under a phase contrast microscope at a 400 fold magnification. Technical details of the method have been described elsewhere (4, see also article by Celis, Kaltoft and Bravo). In this article, map** of early Simian Virus 40 (SV40) specific functions and studies on the activity of purified SV40 T-antigen and T-antigen related proteins are used to demonstrate some aspects of the microinjection technique.
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References
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Graessmann, A., Graessmann, M., Mueller, C. (1980). Biological Activity of Simian Virus 40 DNA Fragments and T-Antigen Tested by Microinjection into Tissue Culture Cells. In: Celis, J.E., Graessmann, A., Loyter, A. (eds) Transfer of Cell Constituents into Eukaryotic Cells. NATO Advanced Study Institutes Series, vol 31. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3707-2_4
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DOI: https://doi.org/10.1007/978-1-4684-3707-2_4
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