33 Result(s)
within
** Lu Li
Microbiology
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Article
Open AccessThe morphology and metabolic changes of Actinobacillus pleuropneumoniae during its growth as a biofilm
Actinobacillus pleuropneumoniae is an important swine respiratory pathogen. Previous studies have suggested that growth as a biofilm is a natural state of A. pleuropneumoniae infection. To understand the survival...
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GrpE and ComD contribute to the adherence, biofilm formation, and pathogenicity of Streptococcus suis
Streptococcus suis is a major bacterial pathogen of swine and an emerging zoonotic agent that has to date resulted in substantial economic losses to the swine industry worldwide, and can cause persistent infectio...
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Analysis of the molecular characteristics of a blaKPC-2-harbouring untypeable plasmid in Serratia marcescens
Serratia marcescens has attracted increasing attention worldwide as a neglected opportunistic pathogen of public health concern, especially due to its antimicrobial resistance features, which usually cause nosoco...
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Open AccessBRD4 inhibitor GNE987 exerts anti-cancer effects by targeting super-enhancers in neuroblastoma
Neuroblastoma (NB) is a common extracranial malignancy with high mortality in children. Recently, super-enhancers (SEs) have been reported to play a critical role in the tumorigenesis and development of NB via...
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Relationship between L-lactate dehydrogenase and multidrug resistance in Staphylococcus xylosus
Staphylococcus xylosus is a gram-positive bacterium that has attracted much attention due to its increasing clinical appearance, frequently associated with serious multidrug resistance cases. L-lactate dehydrogen...
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A versatile toolbox for CRISPR-based genome engineering in Pichia pastoris
Pichia pastoris has gained much attention as a popular microbial cell factory for the production of recombinant proteins and high-value chemicals from laboratory to industrial scale. However, the lack of convenie...
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Open AccessImproving CoQ10 productivity by strengthening glucose transmembrane of Rhodobacter sphaeroides
Several Rhodobacter sphaeroides have been widely applied in commercial CoQ10 production, but they have poor glucose use. Strategies for enhancing glucose use have been widely exploited in R. sphaeroides. Neverthe...
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Open AccessAnalysis of chicken macrophage functions and gene expressions following infectious bronchitis virus M41 infection
Infectious bronchitis virus (IBV) is a pathogenic coronavirus with high morbidity and mortality in chicken breeding. Macrophages with normal biofunctions are essential for host immune responses. In this study,...
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Overexpression of the regulatory subunit of protein kinase A increases heterologous protein expression in Pichia pastoris
Translational regulation plays an important role in protein synthesis. Our goal was to screen translation-related factors to improve heterologous protein expression in Pichia pastoris.
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Multiplex gene editing and large DNA fragment deletion by the CRISPR/Cpf1-RecE/T system in Corynebacterium glutamicum
Corynebacterium glutamicum is an essential industrial strain that has been widely harnessed for the production of all kinds of value-added products. Efficient multiplex gene editing and large DNA fragment deletio...
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Article
Enhancing the substrate tolerance of DszC by a combination of alanine scanning and site-directed saturation mutagenesis
The biodesulfurization 4S pathway can specifically desulfurize an aromatic S heterocyclic compound (which is difficult to desulfurize by hydrodesulfurization) and maintain the integrity of its combustion value...
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A kinetic model to optimize and direct the dose ratio of Dsz enzymes in the 4S desulfurization pathway in vitro and in vivo
To enhance the biodesulfurization rate using a kinetic model that directs the ratio of Dsz enzymes.
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Open AccessMetabolic engineering of Bacillus amyloliquefaciens for enhanced production of S-adenosylmethionine by coupling of an engineered S-adenosylmethionine pathway and the tricarboxylic acid cycle
S-Adenosylmethionine (SAM) is a critical cofactor involved in many biochemical reactions. However, the low fermentation titer of SAM in methionine-free medium hampers commercial-scale production. The SAM synthe.....
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Open AccessEnhancing co-translational folding of heterologous protein by deleting non-essential ribosomal proteins in Pichia pastoris
Translational regulation played an important role in the correct folding of heterologous proteins to form bioactive conformations during biogenesis. Translational pausing coordinates protein translation and co...
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Open AccessImproved xylose tolerance and 2,3-butanediol production of Klebsiella pneumoniae by directed evolution of rpoD and the mechanisms revealed by transcriptomics
The biological production of 2,3-butanediol from xylose-rich raw materials from Klebsiella pneumoniae is a low-cost process. RpoD, an encoding gene of the sigma factor, is the key element in global transcription ...
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Article
Diversity and community structure of ammonia oxidizers in a marsh wetland of the northeast China
As an interface of terrestrial and aquatic ecosystems, wetland is a hotspot of the global nitrogen cycle. Ammonia oxidation is an essential part of the nitrogen cycle and is conducted by ammonia-oxidizing arch...
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Membrane technology for surface water treatment: advancement from microfiltration to membrane bioreactor
Following the rapid proliferation of organic pollutants in the surface water, the application of microfiltration technology has been extensively studied for its treatment since the 1990s. Given that the conven...
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Cloning and Stress-Induced Expression Analysis of Calmodulin in the Antarctic Alga Chlamydomonas sp. ICE-L
Calmodulin (CaM) is a Ca2+-binding protein that plays a role in several Ca2+ signaling pathways, which dynamically regulates the activities of hundreds of proteins. The ice alga Chlamydomonas sp. ICE-L, which has...
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Open AccessGenome-wide comparative analysis of putative Pth11-related G protein-coupled receptors in fungi belonging to Pezizomycotina
G-protein coupled receptors (GPCRs) are the largest family of transmembrane receptors in fungi, where they play important roles in signal transduction. Among them, the Pth11-related GPCRs form a large and dive...
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Article
Production of rhamnolipids with a high specificity by Pseudomonas aeruginosa M408 isolated from petroleum-contaminated soil using olive oil as sole carbon source
Rhamnolipids (RLs) represent a very promising class of biosurfactants that have many applications as biocompatible and biodegradable emulsifiers. We have isolated a Pseudomonas aeruginosa strain, denoted strain M...
