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  1. No Access

    Article

    Identification by comparative transcriptomics of core regulatory genes for higher alcohol production in a top-fermenting yeast at different temperatures in beer fermentation

    Undesirable flavor caused by excessive higher alcohols restrains the development of the wheat beer industry. To clarify the regulation mechanism of the metabolism of higher alcohols in wheat beer brewing by th...

    Zhong-Guan Sun, Meng-Qi Wang, Ya-** Wang in Applied Microbiology and Biotechnology (2019)

  2. Article

    Open Access

    Improved xylose tolerance and 2,3-butanediol production of Klebsiella pneumoniae by directed evolution of rpoD and the mechanisms revealed by transcriptomics

    The biological production of 2,3-butanediol from xylose-rich raw materials from Klebsiella pneumoniae is a low-cost process. RpoD, an encoding gene of the sigma factor, is the key element in global transcription ...

    Xue-Wu Guo, Yu Zhang, Lu-Lu Li, **ang-Yu Guan, Jian Guo in Biotechnology for Biofuels (2018)

  3. No Access

    Article

    Genetic engineering to alter carbon flux for various higher alcohol productions by Saccharomyces cerevisiae for Chinese Baijiu fermentation

    Higher alcohols significantly influence the quality and flavor profiles of Chinese Baijiu. ILV1-encoded threonine deaminase, LEU1-encoded α-isopropylmalate dehydrogenase, and LEU2-encoded β-isopropylmalate dehydr...

    Wei Li, Shi-Jia Chen, Jian-Hui Wang in Applied Microbiology and Biotechnology (2018)

  4. No Access

    Article

    Reduced production of diacetyl by overexpressing BDH2 gene and ILV5 gene in yeast of the lager brewers with one ILV2 allelic gene deleted

    Diacetyl causes an unwanted buttery off-flavor in lager beer. The production of diacetyl is reduced by modifying the metabolic pathway of yeast in the beer fermentation process. In this study, BDH2 and ILV5 genes...

    Ting-Ting Shi, ** Li, Shi-Jia Chen in Journal of Industrial Microbiology & Biote… (2017)

  5. No Access

    Article

    Diacetyl content reduction in industrial brewer’s yeast through ILV2 disruption and BDH1 expression

    Diacetyl is crucial to beer maturation because of its characteristic buttery taste. New industrial brewer’s yeast strains, including single ILV2 allele disruption (QI2), single ILV2 allele disruption and BDH1 ove...

    Ting-Ting Shi, Xue-Wu Guo, ** Li, Zheng Zhou in European Food Research and Technology (2016)

  6. No Access

    Article

    Reduced production of ethyl carbamate for wine fermentation by deleting CAR1 in Saccharomyces cerevisiae

    Ethyl carbamate (EC), a pluripotent carcinogen, is mainly formed by a spontaneous chemical reaction of ethanol with urea in wine. The arginine, one of the major amino acids in grape musts, is metabolized by ar...

    Xue-Wu Guo, Yuan-Zi Li, Jian Guo, Qing Wang in Journal of Industrial Microbiology & Biote… (2016)