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Article
Efficient synthesis of 2-phenylethanol from L-phenylalanine by engineered Bacillus licheniformis using molasses as carbon source
2-Phenylethanol is a valuable flavoring agent with many applications. Although the bioproduction of 2-phenylethanol has been achieved by microbial fermentation, the low titer and high cost hinder its industria...
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Article
Promoting microbial utilization of phenolic substrates from bio-oil
The economic viability of the biorefinery concept is limited by the valorization of lignin. One possible method of lignin valorization is biological upgrading with aromatic-catabolic microbes. In conjunction, ...
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Article
Open AccessMetabolic engineering of Bacillus amyloliquefaciens for enhanced production of S-adenosylmethionine by coupling of an engineered S-adenosylmethionine pathway and the tricarboxylic acid cycle
S-Adenosylmethionine (SAM) is a critical cofactor involved in many biochemical reactions. However, the low fermentation titer of SAM in methionine-free medium hampers commercial-scale production. The SAM synthe.....
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Solid-State Anaerobic Digestion for Waste Management and Biogas Production
Solid-state anaerobic digestion (SS-AD) is commonly used to treat feedstocks with high solid content such as municipal solid waste and lignocellulosic biomass. Compared to liquid state anaerobic digestion (LS-...
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Article
Ultrasonic-assisted extraction of squalene and vitamin E based oil from Zizyphi Spinosae Semen and evaluation of its antioxidant activity
Zizyphi Spinosae Semen (ZSS) is an herbal medicine with a variety of biological activities. In this study, the oil fraction of ZSS was extracted using an ultrasonic assisted extraction (UAE) method and the value ...
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Article
Open AccessRewiring glycerol metabolism for enhanced production of poly-γ-glutamic acid in Bacillus licheniformis
Poly-γ-glutamic acid (γ-PGA) is a natural polymer with great potential applications in areas of agriculture, industry, and pharmaceutical. The biodiesel-derived glycerol can be used as an attractive feedstock ...
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Article
Comprehensive determination of nine polyphenols in Polygoni Avicularis Herba with a new HPLC–DAD method and their correlation with the antioxidant activities
A simple high performance liquid chromatography (HPLC) method was established for identification and quantification of nine polyphenols (gallic acid, chlorogenic acid, caffeic acid, myricetrin, hyperoside, lut...
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Article
Open AccessBiogenic amines analysis and microbial contribution in traditional fermented food of Douchi
Biogenic amines (BAs) have been reported to threaten the Douchi safety, while the BAs formation mechanism and corresponding control method have not been clarified for Douchi. The present study aims to investig...
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Article
Improvement of glycerol catabolism in Bacillus licheniformis for production of poly-γ-glutamic acid
Bacillus licheniformis WX-02 is a well-studied strain to produce poly-γ-glutamic acid (γ-PGA) with numerous applications. This study is to improve WX-02 strain’s capability of assimilatin...
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Article
Damage to the microbial cell membrane during pyrolytic sugar utilization and strategies for increasing resistance
Lignocellulosic biomass is an appealing feedstock for the production of biorenewable fuels and chemicals, and thermochemical processing is a promising method for depolymerizing it into sugars. However, trace c...
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Article
Open AccessDeciphering Clostridium metabolism and its responses to bioreactor mass transfer during syngas fermentation
This study used 13C tracers and dynamic labeling to reveal metabolic features (nutrients requirements, pathway delineation and metabolite turnover rates) of Clostridium carboxidivorans P7, a model strain for indu...
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Article
Open AccessA novel approach to improve poly-γ-glutamic acid production by NADPH Regeneration in Bacillus licheniformis WX-02
Poly-γ-glutamic acid (γ-PGA) is an important biochemical product with a variety of applications. This work reports a novel approach to improve γ-PGA through over expression of key enzymes in cofactor NADPH gen...
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Article
Open AccessEngineering Bacillus licheniformis for the production of meso-2,3-butanediol
2,3-Butanediol (2,3-BD) can be used as a liquid fuel additive to replace petroleum oil, and as an important platform chemical in the pharmaceutical and plastic industries. Microbial production of 2,3-BD by Bacill...
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Article
Utilization of pyrolytic substrate by microalga Chlamydomonas reinhardtii: cell membrane property change as a response of the substrate toxicity
Acetic acid derived from fast pyrolysis of lignocellulosic biomass is a promising substrate for microalgae fermentation for producing lipid-rich biomass. However, crude pyrolytic acetic acid solution contains ...
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Article
Production of biorenewable styrene: utilization of biomass-derived sugars and insights into toxicity
Fermentative production of styrene from glucose has been previously demonstrated in Escherichia coli. Here, we demonstrate the production of styrene from the sugars derived from lignocellulosic biomass depolymeri...
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Article
Open AccessEffects of the surface physico-chemical properties and the surface textures on the initial colonization and the attached growth in algal biofilm
Algal biofilm reactors represent a promising cultivation system that can economically produce biomass without the need for expensive harvesting operations. A critical component of algal biofilm systems is the ...
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Article
Biofilm-based algal cultivation systems
Biofilm-based algal cultivation has received increased attention as a potential platform for algal production and other applications such as wastewater treatment. Algal biofilm cultivation systems represent an...
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Article
Improvement of lichenysin production in Bacillus licheniformis by replacement of native promoter of lichenysin biosynthesis operon and medium optimization
Lichenysin is a biodegradable surfactant with huge potential for recovering crude oil from the oil reservoir. The current production of lichenysin is made through fermentation from wild strain of Bacillus licheni...
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Article
An experimental investigation on the multiphase flows and turbulent mixing in a flat-panel photobioreactor for algae cultivation
Kee** an appropriate mixing state of the multiphase flows in photobioreactors (PBRs) is a key issue for the optimal design and operation of the PBRs. In the present study, an experimental investigation is co...
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Article
Open AccessDeletion of meso-2,3-butanediol dehydrogenase gene bud C for enhanced D-2,3-butanediol production in Bacillus licheniformis
D-2,3-butanediol has many industrial applications such as chiral reagents, solvents, anti-freeze agents, and low freezing point fuels. Traditional D-2,3-butanediol producing microorganisms, such as Klebsiella pne...
