11 Result(s)
within
Protocol
Wayne F. Patton
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Protocol
Detection of Glycoproteins in Gels and Blots
As we become more aware of the significance of posttranslational modifications, such as glycosylation, in the production of recombinant proteins and in the proteomic studies of development and disease, techniq...
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Protocol
Background-Free Protein Detection in Polyacrylamide Gels and on Electroblots Using Transition Metal Chelate Stains
Electrophoretically separated proteins may be visualized using organic dyes such as Ponceau Red, Amido Black, Fast Green, or most commonly Coomassie Brilliant Blue (1,2). Alternatively, sensitive detection met...
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Protocol
Quantitative Analysis of Protein Phosphorylation Status and Protein Kinase Activity on Microarrays Using Pro-Q− Diamond Dye Technology
The human genome is estimated to contain 30,000 to 75,000 genes, but as a result of alternative mRNA splicing and protein posttranslational modifications, the human proteome may contain a million or more prote...
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Protocol
Multiplexed Proteomics Technology for the Fluorescence Detection of Phosphorylation and Protein Expression Levels Using Pro-Q® Diamond and SYPRO® Ruby Dyes
The reversible phosphorylation of serine, threonine, and tyrosine residues is arguably one of the most important covalent posttranslational modifications regulating the functional status of proteins in eukaryo...
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Protocol
Sensitive Quantitative Fluorescence Detection of Proteins in Gels Using SYPRO® Ruby Protein Gel Stain
The fluorescent, noncovalent staining method, using SYPRO® Ruby dye resembles colorimetric staining procedures, such as colloidal Coomassie Blue dye staining. The dye provides noncovalent staining and is appli...
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Protocol
Rapid, Sensitive Detection of Proteins in Minigels With Fluorescent Dyes
The advent of polyacrylamide gel electrophoresis (PAGE) in the minigel format (i.e., gel dimensions in the range of 6 × 9 cm or 8 × 8 cm × 0.75 to 1.5 mm thick), the widespread use of precast minigels, and the...
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Protocol
Multiplexed Proteomics Technology for the Fluorescence Detection of Glycoprotein Levels and Protein Expression Levels Using Pro-Q® Emerald and SYPRO® Ruby Dyes
Protein glycosylation is increasingly being recognized as one of the most prominent posttranslational modifications associated with malignant transformation and tumorogenesis as well as cell differentiation. T...
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Protocol
Detection of Glycoproteins in Gels and Blots
As we become more aware of the significance of posttranslational modifications, such as glycosylation, in the production of recombinant proteins and in the proteomic studies of development and disease, techniq...
-
Protocol
Background-Free Protein Detection in Polyacrylamide Gels and on Electroblots Using Transition Metal Chelate Stains
Electrophoretically separated proteins may be visualized using organic dyes such as Ponceau Red, Amido Black, Fast Green, or most commonly Coomassie Brilliant Blue (1,2). Alternatively, sensitive detection method...
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Protocol
Protein Detection Using Reversible Metal Chelate Stains
Electrophoretically separated proteins may be visualized using organic dyes, such as Ponceau red, Amido black, fast green, or most commonly Coomassie Brilliant Blue (1,2). Alternatively, sensitive detection metho...
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Protocol
Image Acquisition in 2-D Electrophoresis
Prevalent methods for visualizing proteins resolved by two-dimensional (2-D) gel electrophoresis include autoradiography, silver staining, and Coomassie brilliant blue staining (1,2. The organic dye Coomassie bri...
