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Article
A left knee wound complication by non-Hodgkins lymphoma in bilateral total knee arthroplasties
A 70-year-old woman with a history of bilateral primary knee osteoarthritis presented with a left knee wound complication, a non-Hodgkins lymphoma, after bilateral total knee arthroplasties. After exploring se...
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Protocol
Protein Detection Using Reversible Metal Chelate Stains
Electrophoretically separated proteins may be visualized using organic dyes, such as Ponceau red, Amido black, fast green, or most commonly Coomassie Brilliant Blue (1,2). Alternatively, sensitive detection metho...
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Protocol
Image Acquisition in 2-D Electrophoresis
Prevalent methods for visualizing proteins resolved by two-dimensional (2-D) gel electrophoresis include autoradiography, silver staining, and Coomassie brilliant blue staining (1,2. The organic dye Coomassie bri...
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Article
Thromboxane-induced neutrophil adhesion to pulmonary microvascular and aortic endothelium is regulated by CD18
Thromboxane (Tx) A2 generation and subsequent selective pulmonary sequestration of neutrophils (PMNs) is characteristic of several forms of the adult respiratory distress syndrome (ARDS). Therefore, we examined P...
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Article
Inflammatory agonists that increase microvascular permeability in vivo stimulate cultured pulmonary microvessel endothelial cell contraction
Bovine pulmonary microvessel endothelial cells grown on a flexible substrate contract upon the addition of angiotensin II, thrombin, bradykinin, and U44069, a stable analogue of thromboxane A2. All these agents p...
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Article
Eicosanoid modulation of stress fibers in cultured bovine aortic endothelial cells
Leukotrienes (LT) B4, LTD4, and thromboxanes (TX) are cytotoxic, and increase microvascular permeability. Because endothelial stress fibers are theorized to be part of the cytoskeletal mechanism by which microves...
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Article
Leukotriene induction of TxB2 in cultured bovine aortic endothelial cells
The leukotrienes (LT) are potent mediators of inflammation, capable of inducing plasma leakage from postcapillary venules and vasoconstriction of terminal arterioles. Some microvascular effects may be attribut...
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Article
Binding, internalization, and degradation of [125I]insulin by cultured bovine aortic endothelial cells: Effects of serotonin
Cultured bovine aortic endothelial cells bind and internalize[125I]insulin and down regulate insulin receptors. Internalized insulin was not degraded significantly and diffused from the endothelial cells. Neither...
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Article
ADP is not Involved in Thrombin-induced Aggregation of Thrombocytes of a Non-mammalian Vertebrate
PLATELETS are aggregated in vivo and in vitro by ADP1,2. Several agents such as thrombin, collagen and certain fatty acids cause aggregation by releasing ADP3. We reported that ADP, 5-hydroxytryptamine and adrena...
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Article
In vitro Studies of Aggregation of Non-mammalian Thrombocytes
NON-MAMMALIAN thrombocytes are nucleated cells which have been shown to have a haemostatic function paralleling that of mammalian platelets. Thrombocytes are active in the promotion of clots1 and clot retraction2