SpringerLink
Log in

Detection of Glycoproteins in Gels and Blots

  • Protocol
The Protein Protocols Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

As we become more aware of the significance of posttranslational modifications, such as glycosylation, in the production of recombinant proteins and in the proteomic studies of development and disease, techniques for the identification and characterization of the oligosaccharides attached to proteins need to be established.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Institutional subscriptions

Similar content being viewed by others

References

  1. Gander J. E. (1984) Gel protein stains: glycoproteins. Meth. Enzymol. 104, 447–451.

    Article  PubMed  CAS  Google Scholar 

  2. Packer, N. H., Wilkins, M. R., Golaz, O., Lawson, M. A., Gooley, A. A., Hochstrasser, D. F., et al. (1996) Characterization of human plasma glycoproteins separated by two-dimensional gel electrophoresis. Bio. Technol. 14, 66–70

    CAS  Google Scholar 

  3. Thornton, D. J., Carlstedt, I., and Sheehan, J. K. (1996) Identification of Glycoproteins on Nitrocellulose Membranes and Gels,Mol. Biotechnol. 5, 171–176.

    Article  PubMed  CAS  Google Scholar 

  4. Devine, P. L. and Warren, J. A. (1990) Glycoprotein detection on Immobilon PVDF transfer membrane using the periodic acid/Schiff reagent,BioTechniques 8, 492–495.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Proteome Systems Ltd., Sydney, Australia

    Nicolle H. Packer

  2. Co-operative Research Centre for Eye Research Technology, Sydney, Australia

    Malcolm S. Ball

  3. Proteome Systems Ltd., Sydney, Australia

    Peter L. Devine

  4. Molecular Probes Inc., Eugene, OR

    Wayne F. Patton

Authors
  1. Nicolle H. Packer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Malcolm S. Ball
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Peter L. Devine
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wayne F. Patton
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of Hertfordshire, Hatfield, UK

    John M. Walker

Rights and permissions

Reprints and permissions

Copyright information

© 2002 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Packer, N.H., Ball, M.S., Devine, P.L., Patton, W.F. (2002). Detection of Glycoproteins in Gels and Blots. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-169-8:761

Download citation

  • DOI: https://doi.org/10.1385/1-59259-169-8:761

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-940-7

  • Online ISBN: 978-1-59259-169-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation