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Protocol
Detection of Glycoproteins in Gels and Blots
As we become more aware of the significance of posttranslational modifications, such as glycosylation, in the production of recombinant proteins and in the proteomic studies of development and disease, techniq...
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Protocol
Detection of Glycoproteins in Gels and Blots
As we become more aware of the significance of posttranslational modifications, such as glycosylation, in the production of recombinant proteins and in the proteomic studies of development and disease, techniq...
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Chapter
Purification and Chemical Characterization of the High Molecular Weight Glycoconjugate Fraction of the Bovine Tear Film and Comparison to Mucins from Other Sources
The tear film is the layer of liquid that covers the surface of the eye, providing protection from invasion and a smooth surface for correct vision.1 It consists of three components: lipid at the air interface, a...
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Protocol
Glycoprotein Detection of 2-D Separated Proteins
The profile of the two-dimensional (2-D) separation of the protein complement (proteome) of eukaryotic cells and tissues typically contains obvious “trains” of spots that differ in pI and/or apparent molecular ma...