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  1. No Access

    Protocol

    PAM-Less CRISPR-SpRY Genome Editing in Plants

    Engineered SpCas9 variant, SpRY, has been demonstrated to facilitate protospacer adjacent motif (PAM) unrestricted targeting of genomic DNA in various biological systems. Here we describe fast, efficient, and ...

    Simon Sretenovic, Xu Tang, Qiurong Ren, Yong Zhang, Yi** Qi in Plant Genome Engineering (2023)

  2. No Access

    Protocol

    Base Editing in Poplar Through an Agrobacterium-Mediated Transformation Method

    CRISPR-Cas9 systems have revolutionized genome editing in plants and facilitated gene knockout and functional genomic studies in woody plants, like poplar. However, in tree species, previous studies have only ...

    Gen Li, Simon Sretenovic, Gary Coleman, Yi** Qi in Plant Genome Engineering (2023)

  3. No Access

    Protocol

    Assembly and Assessment of Prime Editing Systems for Precise Genome Editing in Plants

    Prime editing, a CRISPR-Cas9-derived precise genome editing strategy, was recently developed to introduce targeted indels and all 12 types of point mutations without DNA double-strand breaks or donor DNA. The ...

    Simon Sretenovic, Changtian Pan, Yi** Qi in CRISPR-Cas Methods (2021)

  4. No Access

    Protocol

    Rapid Vector Construction and Assessment of BE3 and Target-AID C to T Base Editing Systems in Rice Protoplasts

    CRISPR-Cas9 has revolutionized the field of genome engineering. Base editing, a new genome editing strategy, was recently developed to engineer nucleotide substitutions. DNA base editing systems use a catalyti...

    Simon Sretenovic, Changtian Pan, Xu Tang… in Rice Genome Engineering and Gene Editing (2021)