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Article
Comparison of fertile and sterile male gametogenesis in Cryptomeria japonica D. Don
Cryptomeria japonica D. Don is one of the most important conifers for forestry; however, the pollinosis caused by this species is the most prevalent allergy in Japan. Male-sterile mutants have attracted attention...
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Article
Functional analysis of SOC1-like and AGL6-like MADS-box genes of the gymnosperm Cryptomeria japonica
We are interested in controlling strobili production in Cryptomeria japonica D. Don by genetic engineering. However, details of the molecular mechanisms governing reproduction in gymnosperms have not been documen...
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Article
Open AccessCharacterization of expressed sequence tags from a full-length enriched cDNA library of Cryptomeria japonica male strobili
Cryptomeria japonica D. Don is one of the most commercially important conifers in Japan. However, the allergic disease caused by its pollen is a severe public health problem in Japan. Since large-scale analysis o...
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Article
Open AccessFunctional annotation of 19,841 Populus nigra full-length enriched cDNA clones
Populus is one of favorable model plants because of its small genome. Structural genomics of Populus has reached a breakpoint as nucleotides of the entire genome have been determined. Reaching the post genome era...
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Article
Expression of the autofluorescent protein, DsRed2, in the recombinants of the ectomycorrhizal basidiomycete, Suillus grevillei, generated by Agrobacterium-mediated transformation
Recombinants were generated from the ectomycorrhizal basidiomycete, Suillus grevillei, through agroinfection using a binary vector carrying the hygromycin B resistance and the autofluorescent protein, DsRed2, mar...
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Article
Agrobacterium-mediated transformation of the ectomycorrhizal basidiomycete Tricholoma matsutake that produces commercially valuable fruit bodies, matsutake
Using agroinfection with a T-DNA vector carrying a hygromycin resistance marker, the recombinants were generated for the first time from the ectomycorrhizal basidiomycete Tricholoma matsutake, which produces comm...
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Article
An improved transformation system for Lombardy poplar (Populus nigra var. italica)
We report an improved transformation system for Lombardy poplar (Populus nigra var. italica). A new binary vector, pBF2, with 11 unique restriction enzyme sites and the normal neomycin phosphotransferase II (NPTI...