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Article
Open AccessCRISPR/Cas9-mediated disruption of CjACOS5 confers no-pollen formation on sugi trees (Cryptomeria japonica D. Don)
Sugi (Cryptomeria japonica D. Don) is an economically important coniferous tree in Japan. However, abundant sugi pollen grains are dispersed and transported by the wind each spring and cause a severe pollen aller...
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Article
Open AccessCRISPR/Cas9-mediated targeted mutagenesis in Japanese cedar (Cryptomeria japonica D. Don)
Cryptomeria japonica (Japanese cedar or sugi) is one of the most important coniferous tree species in Japan and breeding programs for this species have been launched since 1950s. Genome editing technology can be ...
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Article
Open AccessDehydroquinate dehydratase/shikimate dehydrogenases involved in gallate biosynthesis of the aluminum-tolerant tree species Eucalyptus camaldulensis
Eucalyptus camaldulensis EcDQD/SDH2 and 3 combine gallate formation, dehydroquinate dehydratase, and shikimate dehydrogenase activities. They are candidates for providing the essential gallate for the biosynthesi...
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Article
Low assimilation efficiency of photorespiratory ammonia in conifer leaves
Glutamine synthetase (GS) localized in the chloroplasts, GS2, is a key enzyme in the assimilation of ammonia (NH3) produced from the photorespiration pathway in angiosperms, but it is absent from some coniferous ...
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Article
Collection of expressed genes from the transition zone of Cryptomeria japonica in the dormant season
Heartwood affects the utility of wood because it differs in some properties compared to sapwood. To regulate heartwood formation, its mechanism must be elucidated. However, the molecular basis underlying heart...
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Article
Open AccessFunctional annotation of 19,841 Populus nigra full-length enriched cDNA clones
Populus is one of favorable model plants because of its small genome. Structural genomics of Populus has reached a breakpoint as nucleotides of the entire genome have been determined. Reaching the post genome era...
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Article
Generation and alteration of norlignans in a transition zone during the drying of a Cryptomeria japonica log
Sugi (Cryptomeria japonica D. Don) produces secondary metabolite norlignans in xylem. Several norlignans are involved in the coloration of heartwood and defense of sapwood against microbial invasion. Their biosyn...
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Article
An improved transformation system for Lombardy poplar (Populus nigra var. italica)
We report an improved transformation system for Lombardy poplar (Populus nigra var. italica). A new binary vector, pBF2, with 11 unique restriction enzyme sites and the normal neomycin phosphotransferase II (NPTI...
