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Article
Reducing radioactive cesium transfer from sawdust media to Pleurotus ostreatus fruiting bodies
The Fukushima Daiichi nuclear power plant was damaged, and radionuclides such as 137Cs were spread into the environment. Three years have passed since the accident, and ingestion of foods contaminated with radioa...
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Article
Open AccessCharacterization of a mycovirus associated with the brown discoloration of edible mushroom, Flammulina velutipes
A mycovirus previously identified in brown discolored fruiting bodies of the cultivated mushroom Flammulina velutipes was characterized. We tentatively named the virus the F. velutipes browning virus (FvBV).
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Article
Transformation of Lyophyllum decastes by particle bombardment
The basidiomycete Lyophyllum decastes was transformed by means of particle bombardment. We isolated five transformants under twelve conditions differing in the two parameters of target distance and helium pressur...
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Article
The fruiting-specific Le.flp1 gene, encoding a novel fungal fasciclin-like protein, of the basidiomycetous mushroom Lentinula edodes
To understand the molecular mechanisms of fruiting body formation of basidiomycetous mushrooms, we have isolated over a 100 of developmentally regulated genes that were specifically transcribed during fruiting...
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Article
Expression of the autofluorescent protein, DsRed2, in the recombinants of the ectomycorrhizal basidiomycete, Suillus grevillei, generated by Agrobacterium-mediated transformation
Recombinants were generated from the ectomycorrhizal basidiomycete, Suillus grevillei, through agroinfection using a binary vector carrying the hygromycin B resistance and the autofluorescent protein, DsRed2, mar...
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Article
Agrobacterium-mediated transformation of the ectomycorrhizal basidiomycete Tricholoma matsutake that produces commercially valuable fruit bodies, matsutake
Using agroinfection with a T-DNA vector carrying a hygromycin resistance marker, the recombinants were generated for the first time from the ectomycorrhizal basidiomycete Tricholoma matsutake, which produces comm...
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Article
Karyotype analysis of interspecific fusants of basidiomycetes by pulsed-field gel electrophoresis
The purpose of this research was to analyze the karyotype of the interspecific fusants of twoPleurotus species. Auxotrophic mutants derived from the cultivated strain ofP. ostreatus andP. cornucopiae were used. P...
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Article
Screening of heterozygous DNA markers in shiitake (Lentinula edodes) using de-dikaryotization via preparation of protoplasts and isolation of four meiotic monokaryons from one basidium
A suitable screening method for heterozygous DNA markers in shiitake,Lentinula edodes (Berk.) Pegler, is reported. Monokaryons were derived from a dikaryon by de-dikaryotization via protoplast formation. Compatib...
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Article
Biodegradation of nonlignocellulosic substances II: physical and chemical properties of sawdust before and after use as artificial soil
The physical properties of sawdust including porosity, water retention, and water drainage were analyzed to prove its suitability for use as an artificial soil in the automatic decomposer-extinguisher (GADE) m...
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Article
Relationship between plasma neutrophil elastase and respiratory index of patients who had undergone cardiac surgery with cardiopulmonary bypass
To evaluate the effects of cardiopulmonary bypass (CPB) on the release of polymorphonuclear leukocyte elastase (PMN-E) and postoperative pulmonary function, the perioperative plasma levels of PMN-E in α1-antitryp...
