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An improved transformation system for Lombardy poplar (Populus nigra var. italica)

  • ORIGINAL ARTICLE
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Journal of Forest Research

Abstract

We report an improved transformation system for Lombardy poplar (Populus nigra var. italica). A new binary vector, pBF2, with 11 unique restriction enzyme sites and the normal neomycin phosphotransferase II (NPTII) gene was constructed for the transformation of Lombardy poplar. Genetically transformed adventitious shoots were directly regenerated after cocultivation of stem segments with Agrobacterium tumefaciens EHA105 that harbored a binary vector with genes for the NPTII and enhanced green fluorescent protein. Successful transformation was confirmed by fluorescence microscopy, immunoblotting and Southern blotting analyses, and resistance to kanamycin and geneticin. This transformation system requires less time than our previous method for the regeneration of transgenic shoots. When explants were incubated on a smedium containing dithiothreitol, the transformation frequency increased to approximately 20%.

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Authors and Affiliations

  1. Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute, Tsukuba, 305-8687, Japan

    Mitsuru Nishiguchi, Kazumasa Yoshida, Takeshi Mohri, Tomohiro Igasaki & Kenji Shinohara

Authors
  1. Mitsuru Nishiguchi
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  2. Kazumasa Yoshida
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  3. Takeshi Mohri
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  4. Tomohiro Igasaki
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  5. Kenji Shinohara
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Correspondence to Mitsuru Nishiguchi.

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Nishiguchi, M., Yoshida, K., Mohri, T. et al. An improved transformation system for Lombardy poplar (Populus nigra var. italica). J For Res 11, 175–180 (2006). https://doi.org/10.1007/s10310-006-0203-1

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  • DOI: https://doi.org/10.1007/s10310-006-0203-1

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