20 Result(s)
within
Article
Longjiang Yu
-
Article
Schistosoma japonicum: The design and experimental evaluation of a multivalent DNA vaccine
The aim of this study was to construct and evaluate the immunity efficacy of the DNA multivalent vaccine pVIVO2SjFABP-23. The vaccine was constructed and produced as follows. Forty BALB/c mice were divided into f...
-
Article
A Magnetospirillum strain WM-1 from a freshwater sediment with intracellular magnetosomes
A helical shaped bacterium capable of producing magnetosomes, designated WM-1, was isolated from freshwater sediment through an improved isolated method that combined magnetic separation and the “race track” m...
-
Article
High-resolution study on the hydrochemical variations caused by the dilution of precipitation in the epikarst spring: an example spring of Landiantang at Nongla, Mashan, China
In addition to rainfall, high-resolution measurements of water level, pH, specific conductivity and temperature of Landiantang epikarst spring at Nongla, Mashan county in Guangxi Province, China were recorded ...
-
Article
Effect of CaCl2 treatment on the changing of drought related physiological and biochemical indexes of Brassica napus
Some experiments revealed that the Ca2+ has a relationship with drought resistance. In this paper, some physiological and biochemical indices were studied in order to analyze the effect of Ca2+ treatment on the d...
-
Article
Lonicera confusa has an anatomical mechanism to respond to calcium-rich environment
Calcium (Ca) is an important nutrient element for plant growth and development. Nevertheless, excessive Ca restricts plant communities in Ca-rich environments. Lonicera confusa DC. is one species adapted to Ca-ri...
-
Article
A new environmentally friendly method for astaxanthin extraction from Xanthophyllomyces dendrorhous
Few environmentally friendly solvents are available to extract food-grade astaxanthin. In this paper, some environmentally friendly solvents, such as lactic acid, ethyl lactate, and ethanol, were employed in c...
-
Article
Δ6-Desaturase from Mortierella alpina: cDNA cloning, expression, and phylogenetic analysis
The open reading frame of the Δ6-desaturase gene was isolated from Mortierella alpina W15 and the gene was cloned into a pPIC3.5K vector. The vector was transformed into Pichia pastoris GS115 and expression was i...
-
Article
Assessment of genetic and epigenetic variation during long-term Taxus cell culture
Gradual loss of secondary metabolite production is a common obstacle in the development of a large-scale plant cell production system. In this study, cell morphology, paclitaxel (Taxol®) biosynthetic ability, and...
-
Article
Identification of a fatty acid ∆6-desaturase gene from the eicosapentaenoic acid-producing fungus Pythium splendens RBB-5
A new full-length cDNA (PsD6) putatively encoding a ∆6-desaturase was cloned from the eicosapentaenoic acid-producing fungus Pythium splendens RBB-5. PsD6 contained an open reading frame of 1380 bp encoding a pro...
-
Article
Properties of magnetite nanoparticles produced by magnetotactic bacteria
The magnetic nanoparticles (magnetite) were prepared through the fermentation of the Magnetospirillum strain WM-1 newly isolated by our group. The samples were characterized by TEM, SAED, XRD, rock magnetic analy...
-
Article
Open AccessNew insights into the genetic networks affecting seed fatty acid concentrations in Brassica napus
Rapeseed (B. napus, AACC, 2n = 38) is one of the most important oil seed crops in the world, it is also one of the most common oil for production of biodiesel. Its oil is a mixture of various fatty acids and diss...
-
Article
Two jasmonate-responsive factors, TcERF12 and TcERF15, respectively act as repressor and activator of tasy gene of taxol biosynthesis in Taxus chinensis
Methyl jasmonate (MeJA) is one of the most effective inducers of taxol biosynthetic genes, particularly the tasy gene. However, the mechanism underlying the regulation of tasy by MeJA is still unknown. In this st...
-
Article
Improvement in the docosahexaenoic acid production of Schizochytrium sp. S056 by replacement of sea salt
Schizochytrium is a marine microalga that requires high concentrations of sea salt for growth, although problems arise with significant amounts of chloride ions in the culture medium, whi...
-
Article
Improved eicosapentaenoic acid production in Pythium splendens RBB-5 based on metabolic regulation analysis
Eicosapentaenoic acid (EPA) is an essential polyunsaturated fatty acid for human beings. At present, the production of commercially available long-chain polyunsaturated fatty acids, mainly from wild-caught oce...
-
Article
Open AccessSynteny analysis of genes and distribution of loci controlling oil content and fatty acid profile based on QTL alignment map in Brassica napus
Deciphering the genetic architecture of a species is a good way to understand its evolutionary history, but also to tailor its profile for breeding elite cultivars with desirable traits. Aligning QTLs from div...
-
Article
Open AccessTranscriptome-wide identification and screening of WRKY factors involved in the regulation of taxol biosynthesis in Taxus chinensis
WRKY, a plant-specific transcription factor family, plays important roles in pathogen defense, abiotic cues, phytohormone signaling, and regulation of plant secondary metabolism. However, little is known about...
-
Article
Open AccessNew different origins and evolutionary processes of AP2/EREBP transcription factors in Taxus chinensis
Taxus spp. produces the anticancer drug, taxol, and hence is planted as an industrial crop in China. APETALA2/ethylene response element binding proteins (AP2/EREBPs) are the key regulators of plant development, g...
-
Article
Open AccessTranscriptional reprogramming strategies and miRNA-mediated regulation networks of Taxus media induced into callus cells from tissues
Taxus cells are a potential sustainable and environment-friendly source of taxol, but they have low survival ratios and slow grow rates. Despite these limitations, Taxus callus cells induced through 6 months of c...
-
Article
Visual analysis of the morphological features and polysaccharide distribution of raw ramie and their influence on degumming
The morphological features of raw ramie and the distribution of polysaccharides on such features were investigated using various microscopes to reveal the pivotal factors that influence microbial degumming eff...
-
Article
Open AccessNew insights into phenotypic heterogeneity for the distinct lipid accumulation of Schizochytrium sp. H016
Schizochytrium sp. is a marine heterotrophic protist and an important sustainable resource for high value-added docosahexaenoic acid in the future. The production of different phenotypes during the continuous sub...
