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Article
A multiplex direct PCR method for the rapid and accurate discrimination of three species of spider mites (Acari: Tetranychidae) in fruit orchards in Bei**g
Spider mites (Acari: Tetranychidae) are polyphagous pests of economic importance in agriculture, among which the two-spotted spider mite Tetranychus urticae Koch has spread widely worldwide as an invasive species...
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Article
Open AccessNitric oxide mediates red light-induced perylenequinone production in Shiraia mycelium culture
Perylenequinones (PQs) from bambusicolous Shiraia fungi serve as excellent photosensitizers for photodynamic therapy. However, the lower yield of PQ production in mycelium cultures is an important bottleneck for ...
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Article
Bamboo polysaccharides elicit hypocrellin A biosynthesis of a bambusicolous fungus Shiraia sp. S9
Hypocrellin A (HA), a fungal perylenequinone from bambusicolous Shiraia species, is a newly developed photosensitizer for photodynamic therapy in cancer and other infectious diseases. The lower yield of HA is an ...
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Article
Open AccessEffects of branched-chain amino acids on Shiraia perylenequinone production in mycelium cultures
Perylenequinones from Shiraia fruiting bodies are excellent photosensitizers and widely used for anti-cancer photodynamic therapy (PDT). The lower yield of Shiraia perylenequinones becomes a significant bottlenec...
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Article
Open AccessContrasting regulation of live Bacillus cereus No.1 and its volatiles on Shiraia perylenequinone production
Fungal perylenequinones (PQs) are a class of photoactivated polyketide mycotoxins produced by plant-associated fungi. Hypocrellins, the effective anticancer photodynamic therapy (PDT) agents are main bioactive...
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Article
Endophytes in Artemisia annua L.: new potential regulators for plant growth and artemisinin biosynthesis
The medicinal plant Artemisia annua L. is presently the sole plant source of artemisinin, the key ingredient of first-line antimalarial drug. As the content of artemisinin in the native plant is very low, the gro...
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Article
Open AccessThe signaling role of extracellular ATP in co-culture of Shiraia sp. S9 and Pseudomonas fulva SB1 for enhancing hypocrellin A production
Adenosine 5′-triphosphate (ATP) plays both a central role as an intracellular energy source, and a crucial extracellular signaling role in diverse physiological processes of animals and plants. However, there ...
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Article
Open AccessNitric oxide donor sodium nitroprusside-induced transcriptional changes and hypocrellin biosynthesis of Shiraia sp. S9
Nitric oxide (NO) is a ubiquitous signaling mediator in various physiological processes. However, there are less reports concerning the effects of NO on fungal secondary metabolites. Hypocrellins are effective...
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Article
Open AccessInducing perylenequinone production from a bambusicolous fungus Shiraia sp. S9 through co-culture with a fruiting body-associated bacterium Pseudomonas fulva SB1
Fungal perylenequinonoid (PQ) pigments from Shiraia fruiting body have been well known as excellent photosensitizers for medical and agricultural uses. The fruiting bodies are colonized by a diverse bacterial com...
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Article
Open AccessEngineering of 3-ketosteroid-∆1-dehydrogenase based site-directed saturation mutagenesis for efficient biotransformation of steroidal substrates
Biosynthesis of steroidal drugs is of great benefit in pharmaceutical manufacturing as the process involves efficient enzymatic catalysis at ambient temperature and atmospheric pressure compared to chemical sy...
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Article
Effect of down-regulating 1-deoxy-d-xylulose-5-phosphate reductoisomerase by RNAi on growth and artemisinin biosynthesis in Artemisia annua L.
1-Deoxy-d-xylulose-5-phosphate reductoisomerase (DXR), an important enzyme in the 2-c-methyl-d-erythritol-4-phosphate (MEP) pathway in plant plastids, provides the basic five-carbon units for isoprenoid biosynthe...
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Article
Efficient degradation of triclosan by an endophytic fungus Penicillium oxalicum B4
Triclosan (TCS), a widely used antimicrobial and preservative agent, is an emerging contaminant in aqueous and soil environment. Microbial degradation of TCS has not been reported frequently because of its inh...
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Article
Cloning and characterization of an expansin gene AbEXP from Achyranthes bidentata
Achyranthes bidentata, a traditional perennial medicine herb in China, contains a rich source of important pharmaceutically active phytoecdysteroids including 20-hydroxyecdysone (20E). Th...
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Article
Open AccessRational design of cholesterol oxidase for efficient bioresolution of cholestane skeleton substrates
Cholesterol oxidase catalyzes the oxidation and isomerization of the cholestane substrates leading to the addition of a hydroxyl group at the C3 position. Rational engineering of the cholesterol oxidase from Pime...
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Article
Open AccessBiochemical analysis and the preliminary crystallographic characterization of d-tagatose 3-epimerase from Rhodobacter sphaeroides
d-Tagatose 3-epimerase epimerizes d-fructose to yield d-psicose, which is a rare sugar that exists in small quantities in nature and is difficult to synthesize chemically. We aim to ex...
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Article
Transcriptomic responses involved in enhanced production of hypocrellin A by addition of Triton X-100 in submerged cultures of Shiraia bambusicola
The addition of surfactant is a useful strategy to enhance the product yield in submerged fermentation process. In this study, we sought to explore the mechanism for the elicitation of Triton X-100 on producti...
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Article
Thiolate/disulfide organic redox couples for efficient organic dye-sensitized solar cells
A series of organic thiolate/disulfide redox couples based on benz-imidazole/othiazole/oxazole have been synthesized and applied to dye-sensitized solar cells (DSCs). Platinum (Pt) and carbon material are intr...
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Article
Molecular characterization of an elicitor-responsive 3-hydroxy-3-methylglutaryl coenzyme A reductase gene involved in oleanolic acid production in cell cultures of Achyranthes bidentata
Achyranthes bidentata contains a rich source of important pharmaceutically active triterpene acids including oleanolic acid (OA) as a major one. 3-Hydroxy-3-methylglutaryl coenzyme A redu...
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Article
Transcriptome sequencing and development of novel genic SSR markers for Dendrobium officinale
Dendrobium officinale is a critically endangered and valuable medicinal herb. In this study, two transcriptome libraries were constructed from 2-year-old stems of two cultivated varieties...
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Article
Multienzymatic cascade synthesis of fucosyloligosaccharide via a two-step fermentation strategy in Escherichia coli
To achieve multienzymatic cascade synthesis of fucosyl oligosaccharide from d-mannose by two-step fermentation pathway in Escherichia coli.