35 Result(s)
within
Article
Isao Karube
Life Sciences
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Article
Open AccessIdentification of G-quadruplex structures that possess transcriptional regulating functions in the Dele and Cdc6 CpG islands
G-quadruplex is a DNA secondary structure that has been shown to play an important role in biological systems. In a previous study, we identified 1998 G-quadruplex-forming sequences using a mouse CpG islands D...
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PCR-Based Ribosomal DNA Detection Technique for Microalga (Heterosigma carterae) Causing Red Tide and Its Application to a Biosensor Using Labeled Probe
A technique for detecting Raphidophycean, a bloom-forming genus of algae, was developed using a specific DNA probe. The design of the probe was based on a sequence polymorphism within the small subunit (SSU) ribo...
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Fructose-2,6-bisphosphate contents were increased in response to salt, water and osmotic stress in leaves of Bruguiera gymnorrhiza by differential changes in the activity of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphate 2-phosphatase
The steady-state level of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphate 2-phosphatase (F6P2K/F26BPase) transcript has been found to be raised in the mangrove Bruguiera gymnorrhiza treated with 500 mM NaCl fo...
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Detection technique of asymmetric RT-PCR-based amplified single-stranded DNA and its application to biosensor for detection of mRNA for cyanobacteria, Anabaena variabilis
A real-time detection technique for mRNA was developed using a specific DNA probe. The design of the probe was based on a sequence polymorphism within the expressed mRNA of Anabaena variabilis PCC 7120 by using t...
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Molecular cloning and characterization of genes encoding BURP domain-containing protein in the mangrove, Bruguiera gymnorrhiza
We cloned and characterized four independent genes encoding BURP domain-containing proteins from mangrove, Bruguiera gymnorrhiza, two as cDNA (BgBDC2 and BgBDC3) and the other two as genomic DNA (S2 and L3). The...
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Molecular characterization and response to salt stress of mRNAs encoding cytosolic Cu/Zn superoxide dismutase and catalase from Bruguiera gymnorrhiza
To analyze the potential of the active oxygen-scavenging system of the cytosol in leaves of salt-stressed Bruguiera gymnorrhiza, we isolated a full-length cDNA encoding a 153-amino-acid sequence of cytosolic Cu/...
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Enhanced eicosapentaenoic acid production by Navicula saprophila
Changes in the eicosapentaenoic acid (EPA) content of Navicula saprophila grown photoautotrophically and mixotrophically with the addition of acetate were examined in terms of their growth progress. Among four...
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Changes in eicosapentaenoic acid content of Navicula saprophila, Rhodomonas salina and Nitzschia sp. under mixotrophic conditions
Three species of microalgae able to produce eicosapentaenoic acid(EPA) were collected from brackish and sea water around Japan. The species were identified as Navicula saprophila, Rhodomonassalina and Nitzschi...
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Continuous two step bioconversion of 4-oxoiso-phorone (OIP) to 4-hydroxy-2,2,6-trimethylcyclo-hexanone (4-HTMCH) by thermophilic bacteria
The continuous two step conversion of 4-oxoisophorone(OIP) to 4-hydroxy-2,2,6-trimethylcyclohexanone(4-HTMCH) via dihydrooxoisophorone(DOIP) was carried out using two types of thermophilic growing cells, Thermomo...
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In vivo fluorometric method for early detection of cyanobacterial waterblooms
A specific method was developed for monitoring the concentration of cyanobacteria (blue-green algae) before waterblooms, based on their characteristics ofin vivo fluorescence. The excitation and emission spectra ...
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Structural characterization of lipid A component of Erwinia carotovora lipopolysaccharide
The chemical structure of the lipid A component of lipopolysaccharide excreted into the liquid medium by the plant pathogenic enterobacterium Erwinia carotovora FERM P-7576 was characterized. It consists of a β-1...
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Article
Sequential two-step conversion of 4-oxoisophorone to 4-hydroxy-2,2,6-trimethylcyclohexanone by thermophilic bacteria
The sequential two-step conversion of 4-oxoisophorone (OIP) to 4-hydroxy-2,2,6-trimethylcyclohexanone (4-HTMCH) via diyhdrooxoisophorone (DOIP) was achieved using two kinds of thermophiles, Thermomonospora curvat...
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Cloning and expression of Citrobacter freundii hydrogenase genes in Escherichia coli
Escherichia coli mutants deficient in hydrogenase activity (Hyd-) were derived from E. coli C600 by mutagenesis with nitrosoguanidine. Hydrogenase activities of mutant strains; HK-2, HK-7, HK-8, HK-16, HK-23, an...
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Production of l-glutamate by immobilized protoplasts
Protoplasts of Brevibacterium flavum cultured in a medium containing 50 μg·l-1 of biotin were prepared with lysozyme and immobilized in matrices of agar-acetylcellulose filters. The immobilized protoplasts were a...
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Cloning and expression of Citrobacter freundii β-isopropylmalate dehydrogenase gene in both Escherichia coli and Bacillus subtilis
β-Isopropylmalate (IPM) dehydrogenase gene of Citrobacter freundii was cloned in both Escherichia coli and Bacillus subtilis. Plasmid pCBL 1 containing C. freundii β-IPM dehydrogenase gene was isolated using E. c...
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Enzyme sensor for hypoxanthine and inosine determination in edible fish
An enzyme sensor for hypoxanthine (Hx) and inosine (HxR), consisting of an enzyme membrane and an oxygen electrode, was constructed, Xanthine oxidase (XO) and nucleoside phosphorylase (NP) were both immobilize...
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Isolation and partial characterization of large plasmids in hydrogen-evolving bacterium Clostridium butyricum
The hydrogen-evolving bacterium Clostridium butyricum IFO 3847 was found to carry three plasmids: pSSK1, pSSK2, and pSSK3. The molecular weights of pSSK1, 2 and 3, determined by agarose gel electrophoresis and el...
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Sulfite ion sensor with use of immobilized organelle
The sulfite ion sensor consisted of immobilized microsomes and an oxygen electrode. When the sensor was inserted into the sample solution, a steady-state current was obtained within 10 min. A linear relationsh...
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Hybrid urea sensor using nitrifying bacteria
A new urea sensor was developed using immobilized nitrifying bacteria isolated from activated sludges and urease. The sensor was composed of a urease-membrane, a cation exchange membrane, and an alkaline bed (...
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Amperometric determination of sodium nitrite by a microbial sensor
A microbial sensor was prepared to determine sodium nitrite. This microbial sensor consisted of immobilized Nitrobacter sp. and an oxygen electrode. When a sample solution containing sodium nitrite was tested, ni...
