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Article
Gibberellin A3 as an epigenetic determinant of global DNA hypo-methylation in tobacco
Gibberellins (GAs) are a large family of tetracyclic diterpenoids, controlling important aspects of growth and development throughout the plant life cycle. To explore the possibility that gibberellin A3 (GA3) sig...
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Article
Concerted evolution rapidly eliminates sequence variation in rDNA coding regions but not in intergenic spacers in Nicotiana tabacum allotetraploid
Nicotiana tabacum (tobacco) is a natural allotetraploid that formed from two diploid progenitors (N. sylvestris—S-genome, N. tomentosiformis—T-genome) within past 0.2 million years. Previ...
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Article
Distribution of 5-methylcytosine residues in 5S rRNA genes in Arabidopsis thaliana and Secale cereale
Bisulfite genomic sequencing was used to localise 5-methylcytosine residues (mC) in 5S rRNA genes of Arabidopsis thaliana and Secale cereale. The maps of mC distribution were compared with the previously publish...
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Article
Transposition of IS10 from the host Escherichia coli genome to a plasmid may lead to cloning artefacts
During recloning of Nicotiana tabacum L. repetitive sequence R8.3 in Escherichia coli, a modified clone that differed from the original by the insertion of an IS10 sequence was unintentionally produced. The inse...
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Article
Comparative analysis of DNA methylation in tobacco heterochromatic sequences
Cytosine methylation levels and susceptibility to drug-induced hypomethylation have been studied in several Nicotiana tabacum (tobacco) DNA repetitive sequences. It has been shown using HapII, MspI, BamHI and Sau...
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Article
Map** of 5-methylcytosine residues in Nicotiana tabacum 5S rRNA genes by genomic sequencing
Genomic sequencing was used to localise 5-methylcytosine residues in individual DNA strands of 5S rRNA genes in tobacco. The density of methylation along the sequence was high in both strands, exceeding the a...
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Article
Meiotic transmission of a hypomethylated repetitive DNA family in tobacco
We have recently shown that hypomethylation of cytosine residues in the HRS60 family of repetitive DNA sequences can be induced with 5-azacytidine (5-azaC) in tobacco tissue cultures. We have also proven that ...
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Article
Session 01 Cell structure and development
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Article
Immunological quantification of recA protein in cell extracts ofE. coli after exposure to chemical mutagens or UV radiation
Increased synthesis of RecA protein is induced inE. coli cells after their damage, the rate of synthesis being dependent on the extent of DNA alterations. The level of the RecA protein was determined inE. coli ce...