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124 Result(s)
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Teruo Okano
English
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Article
Endothelial cell behavior inside myoblast sheets with different thickness
Using a cell sheet stacking method, we developed an in vitro culture system in which green fluorescent protein expressing human umbilical vein endothelial cells (GFP-HUVECs) were cultured under human skeletal ...
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Article
Successive grafting of PHEMA and PIPAAm onto cell culture surface enables rapid cell sheet recovery
Fabrication of functional tissue constructs from designed three-dimensional structure of cells using layered method of cultured cell sheets could prove to be an attractive approach to tissue engineering. Rapid...
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Article
Open AccessIn Vitro Engineering of Vascularized Tissue Surrogates
In vitro scaling up of bioengineered tissues is known to be limited by diffusion issues, specifically a lack of vasculature. Here, we report a new strategy for preserving cell viability in three-dimensional tissu...
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Article
Synthesis of terminal-functionalized thermoresponsive diblock copolymers using biodegradable macro-RAFT agents
Well-defined multifunctional diblock copolymers with terminal functional groups were synthesized by the combination of cationic ring opening polymerization (CROP) and reversible addition-fragmentation chain tr...
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Article
Open AccessIn vitro fabrication of functional three-dimensional tissues with perfusable blood vessels
Artificially engineered tissues may have many therapeutic applications but complex tissues are hard to create in vitro. Here, Okano and colleagues report the production of functional cardiac tissue sheets with pe...
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Article
Development of Eczematous Symptoms by the Implanted Breast Prosthesis
A patient with an unidentified implanted breast prosthesis experienced eczematous symptoms 3 years after implantation, although prosthesis rapture and complications such as capsular contraction, hematoma, and ...
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Article
Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro
The fabrication of 3D tissues retaining the original functions of tissues/organs in vitro is crucial for optimal tissue engineering and regenerative medicine. The fabrication of 3D tissues also contributes to the...
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Article
Myocardial tissue engineering: toward a bioartificial pump
Regenerative therapies, including cell injection and bioengineered tissue transplantation, have the potential to treat severe heart failure. Direct implantation of isolated skeletal myoblasts and bone-marrow-d...
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Article
Tissue engineered myoblast sheets improved cardiac function sufficiently to discontinue LVAS in a patient with DCM: report of a case
Dilated cardiomyopathy (DCM) is a heart muscle disease characterized by progressive heart failure, and is a leading cause of mortality and morbidity. Recently, cellular therapy for end-stage heart failure has ...
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Article
Reproducible subcutaneous transplantation of cell sheets into recipient mice
Perfecting tissue engineering and cell sheet transplantation is an important step toward realizing regenerative medicine and is a growing area of research. Before being applied to clinical settings, it is impo...
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Article
“Deep-media culture condition” promoted lumen formation of endothelial cells within engineered three-dimensional tissues in vitro
In the field of tissue engineering, the induction of microvessels into tissues is an important task because of the need to overcome diffusion limitations of oxygen and nutrients within tissues. Powerful method...
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Article
Assessment of cell sheets derived from human periodontal ligament cells: a pre-clinical study
Periodontal-ligament-derived cells (PDL cells) have stem-cell-like properties and, when implanted into periodontal defects in vivo, can induce periodontal regeneration including the formation of new bone, ceme...
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Article
Cell Attachment–Detachment Control on Temperature-Responsive Thin Surfaces for Novel Tissue Engineering
Temperature-responsive intelligent surfaces, prepared by the modification of an interface mainly with poly(N-isopropylacrylamide) and its derivatives, have been investigated. Such surfaces exhibit temperature-res...
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Book
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Chapter
Stimuli-Responsive Hydrogels and Their Application to Functional Materials
Many kinds of stimuli-responsive polymer gels that respond to the change in their surroundings such as solvent composition, temperature, pH, and supply of electric field have been developed. They are of intere...
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Article
Dual Temperature- and pH-Responsive Fluorescence Molecular Probe for Cellular Imaging Utilizing a PNIPAAm-Fluorescein Copolymer
The thermally controlling fluorescence intensity of a molecular probe for cellular imaging has been investigated. A reversible temperature-induced phase transition of N-isopropylacrylamide/fluorescein copolymers ...
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Chapter and Conference Paper
Fabrication of Three-Dimensional Tissues with Perfused Microchannels
Recently, researchers have challenged to create three-dimensional (3-D) tissues with tissue engineering technology in order to establish in vitro models and new therapy for damaged organ. We have developed cel...
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Chapter
On-Off Switching Properties of ultra thin Intelligent Temperature-Responsive Polymer Modified Surface
Intelligent temperature-responsive polymers are currently utilized in a variety of fields within engineering and medicine. Particularly, special attention has been paid to the temperature-responsive polymer, p...
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Article
Fibroblast sheets co-cultured with endothelial progenitor cells improve cardiac function of infarcted hearts
We have already confirmed that cell sheet transplantation can improve damaged heart function via continuous cytokine secretion. In this study, we hypothesized that cytokine-secreting cell sheets co-cultured wi...
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Article
Microfluidic devices for size-dependent separation of liver cells
Liver is composed of various kinds of cells, including hepatic parenchymal cells (hepatocytes) and nonparenchymal cells, and separation of these cells is essential for cellular therapies and pharmacological an...
