174 Result(s)
within
Chun-** Peng
Life Sciences
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Article
Open AccessDetection of quantitative trait loci affecting haematological traits in swine via genome scanning
Haematological traits, which consist of mainly three components: leukocyte traits, erythrocyte traits and platelet traits, play extremely important role in animal immune function and disease resistance. But kn...
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NYGGF4 homologous gene expression in 3T3-L1 adipocytes: regulation by FFA and adipokines
NYGGF4 is a novel gene that is abundantly expressed in the adipose tissue of obese subjects and is involved in insulin resistance. In the present study, the mRNA expression of NYGGF4 homologous genes was examined...
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Article
Selective chemical labeling reveals the genome-wide distribution of 5-hydroxymethylcytosine
Song et al. present the first method for global analysis of 5-hydroxymethylcytosine, a recently identified epigenetic modification in mammalian cells. They use a bacteriophage-derived enzyme to tag the hydroxymet...
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Article
CDK1-dependent phosphorylation of EZH2 suppresses methylation of H3K27 and promotes osteogenic differentiation of human mesenchymal stem cells
The Polycomb group protein EZH2 promotes trimethylation of histone H3K27 and gene silencing. Cdk1 is found to phosphorylate EZH2 to inhibit its methyltransferase activity, affecting EZH2-target genes involved ...
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Article
Protection of CHO cells by transfer of survivin driven by ovarian-specific promoter OSP-2
Chemotherapy is the major therapy for cancer in clinic. However, chemotherapeutic agents can harm the other tissues/organs besides cancer. Thus, there are great interests in protecting the innocents by the tra...
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Overexpression of a 10-deacetylbaccatin III-10 β-O-acetyltransferase gene leads to increased taxol yield in cells of Taxus chinensis
Taxus chinensis suspension cells were transformed by the Agrobacterium-mediated transformation method to overexpress the dbat gene coding for 10-deacetylbaccatin III-10 β-O-acetyltransferase, a key enzyme for tax...
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Expression of Trichoderma viride endoglucanase III in the larvae of silkworm, Bombyx mori L. and characteristic analysis of the recombinant protein
Endoglucanase is a part of cellulase which hydrolyzes cellulose into glucose. In this study, we cloned endoglucanase III (EG III) gene from Trichoderma viride strain AS 3.3711 using a PCR-based exon splicing meth...
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Article
Surface display of the 20-kDa N-terminal fragment of anthrax protective antigen based on attenuated recombinant Bacillus anthracis
Extracellular antigen 1 (EA1), a major component of the Bacillus anthracis surface layer (S-layer), was used as a fusion partner for the expression of heterologous antigen. A recombinant B. anthracis strain was c...
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Article
Open AccessGenome-wide patterns of genetic variation in sweet and grain sorghum (Sorghum bicolor)
Sorghum (Sorghum bicolor) is globally produced as a source of food, feed, fiber and fuel. Grain and sweet sorghums differ in a number of important traits, including stem sugar and juice accumulation, plant height...
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Article
Distribution and expression characteristics of triterpenoids and OSC genes in white birch (Betula platyphylla suk.)
Betulin and oleanolic acids (pentacyclic triterpenoid secondary metabolites) have broad pharmacological activities and can be potentially used for the development of anti-cancer and anti-AIDS drugs. In this st...
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Open AccessTranscriptional profile of Taxus chinensis cells in response to methyl jasmonate
Methyl jasmonate (MeJA) has been successfully used as an effective elicitor to enhance production of taxol and other taxanes in cultured Taxus cells. However the mechanism of MeJA-mediated taxane biosynthesis rem...
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Article
Tet-assisted bisulfite sequencing of 5-hydroxymethylcytosine
A complete understanding of the potential function of 5-hydroxymethylcytosine (5-hmC), a DNA cytosine modification in mammalian cells, requires an accurate single-base resolution sequencing method. Here we des...
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Article
Open AccessWhole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes
The opportunistic enterobacterium, Morganella morganii, which can cause bacteraemia, is the ninth most prevalent cause of clinical infections in patients at Changhua Christian Hospital, Taiwan. The KT strain of M...
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Article
Molecular characterization and phylogenetic analysis of infectious bursal disease viruses isolated from chicken in South China in 2011
Infectious bursal disease virus (IBDV) is a double-stranded RNA virus that causes immunosuppressive disease in young chickens. Thousands of cases of IBDV infection are reported each year in South China, and th...
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Article
Subtelomeric hotspots of aberrant 5-hydroxymethylcytosine-mediated epigenetic modifications during reprogramming to pluripotency
Mammalian somatic cells can be directly reprogrammed into induced pluripotent stem cells (iPSCs) by introducing defined sets of transcription factors. Somatic cell reprogramming involves epigenomic reconfigura...
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Transcriptome analysis of candidate genes and signaling pathways associated with light-induced brown film formation in Lentinula edodes
High-throughput Illumina RNA-seq was used for deep sequencing analysis of the transcriptome of poly(A)+ RNA from mycelium grown under three different conditions: 30 days darkness (sample 118), 80 days darkness...
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Article
Screening of high α-arbutin producing strains and production of α-arbutin by fermentation
A mutant Xanthomonas maltophilia BT-112 with high α-anomer-selective glycosylation activity was screened by a series of mutation methods including UV light, N-methyl-N-nitro-N-nitroso-guanidine tr...
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Article
Open AccessGlycolipid metabolic status of overweight/obese adolescents aged 9- to 15-year-old and the BMI-SDS/BMI cut-off value of predicting dyslipidemiain boys, Shanghai, China: a cross-sectional study
The prevalence of adolescents’ obesity and overweight has dramatically elevated in China. Obese children were likely to insulin resistance and dyslipidemia, which are risk factors of cardiovascular diseases. H...
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Open AccessAspidistra crassifila (Asparagaceae), a new species from Guangxi, China
Aspidistra crassifila Yan Liu & C.-I Peng, a new species of the Asparagaceae from Guangxi Zhuang Autonomous Region, China, is described and illustrated.
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Article
Zinc fertilizer placement affects zinc content in maize plant
Adequate zinc (Zn) in maize (Zea mays L.) is required for obtaining Zn-enriched grain and optimum yield. This study investigated the impact of varying Zn fertilizer placements on Zn accumulation in maize plant.
