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Purification and Some Kinetic Characteristics of Succinate Dehydrogenase Isoenzymes from Maize Leaves under Salt Stress
Highly purified succinate dehydrogenase (SDH) preparations were obtained using multistage purification. Ion-exchange chromatography on DEAE-Sephacel...
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Light Regulation of Tricarboxylic Acid Cycle Isoenzymes in Plants
AbstractLight regulation of the tricarboxylic acid cycle (TCA) in green plant leaves is carried out by active forms of phytochrome and cryptochrome...
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The importance of polymorphisms in the genes encoding glutathione S-transferase isoenzymes in development of selected cancers and cardiovascular diseases
Glutathione S-transferases are a family of enzymes, whose main role is to detoxify cells from many exogenous factors, such as xenobiotics or...
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Two-Dimensional Gel Electrophoresis: Discovering Isoenzymes for Mycoremediation
Exploring catabolic pathways and the enzymes involved in these processes allows enhancing mycoremediation treatments. LaccaseLaccases and lipolytic...
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Purification of Constitutive Isoenzymes of Succinate Dehydrogenase from Zea mays Scutellum in the Homogeneous State and the Study of Their Characteristics
AbstractThe use of a modified, four-stage purification scheme allowed us to obtain electrophoretically homogeneous preparations of constitutive forms...
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Alkaline Phosphatases: Biochemistry, Functions, and Measurement
Alkaline phosphatases (ALPs) are a group of isoenzymes, situated on the external layer of the cell membrane; they catalyze the hydrolysis of organic...
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Identification of the Inhibition Effects of Some Natural Antiproliferative Agents on CA-I, CA-II, and AChE Activities Isolated from Human Erythrocytes by Kinetic and Molecular Docking Studies
AbstractNowadays the determination of inhibitors of carbonic anhydrase isoenzymes (CAs) have become one of the main goals of drug design studies, and...
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Purification, characterization, and gene cloning of two laccase isoenzymes (Lac1 and Lac2) from Trametes hirsuta MX2 and their potential in dye decolorization
In this study, two laccase isoenzymes (Lac1 and Lac2) from the culture supernatant of Trametes hirsuta MX2 were purified, and the genes ( Lac1 and Lac2 ...
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Investigation of Potential Effects of Some Indole Compounds on the Glutathione S-Transferase Enzyme
AbstractGlutathione S-transferases (GSTs) belong to the superfamily of multifunctional detoxification isoenzymes with an important role in cellular...
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Pattern of ROS generation and interconversion on wet stigmas in basal and divergent angiosperms
The active participation of ROS in the reproductive physiology of flowering plants is well-documented in model species, mainly plants with dry...
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Curcumin-based-fluorescent probes targeting ALDH1A3 as a promising tool for glioblastoma precision surgery and early diagnosis
Glioblastoma (GBM) is the most aggressive primary brain tumour for which both effective treatments and efficient tools for an early-stage diagnosis...
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Synthesis, molecular docking and molecular dynamics simulations, drug-likeness studies, ADMET prediction and biological evaluation of novel pyrazole-carboxamides bearing sulfonamide moiety as potent carbonic anhydrase inhibitors
Pyrazoles are unique bioactive molecules with a versatile biological profile and they have gained an important place on pharmaceutical chemistry....
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Unsymmetrical Cysteine Disulfides as Carbonic Anhydrase Inhibitors
AbstractA small library of unsymmetrical cysteine disulfides as four aliphatic, three aromatic and one heteroaromatic were evaluated for their...
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Glutathione Transferases are Involved in Salicylic Acid-Induced Transcriptional Reprogramming
Salicylic acid (SA) plays a crucial role not only in defence against pathogen attacks, but also in abiotic stress responses. Recently, some key steps...
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Applications of Molecular Biology in Agriculture
Detailed knowledge of the processes involved in the central dogma of molecular biology has enabled the direct or indirect use of DNA in molecular...
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A Nonradioactive Method to Measure Hyaluronan Synthase Activity
Hyaluronan (HA) is a component of the extracellular matrix that is involved in many physiological and pathological processes. As HA modulates several...
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Novel bis-ureido-substituted sulfaguanidines and sulfisoxazoles as carbonic anhydrase and acetylcholinesterase inhibitors
AbstractTo discover alternative substances to compounds used to treat many diseases, especially treating Alzheimer’s disease (AD) and Parkinson’s...
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Laccase Isoenzymes of Trametes hirsuta LE-BIN072: Degradation of Industrial Dyes and Secretion under the Different Induction Conditions
It is well-known that various oxidative enzymes of wood-degrading fungi are widely demanded in various fields of biotechnology. Laccases attract...
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A novel laccase from Trametes polyzona with high performance in the decolorization of textile dyes
Laccases are multicopper oxidases able to oxidize several phenolic compounds and find application in numerous industrial applications. Among laccase...
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Identification of steroid C27 monooxygenase isoenzymes involved in sterol catabolism and stepwise pathway engineering of Mycobacterium neoaurum for improved androst-1,4-diene-3,17-dione production
Cholesterol oxidase, steroid C27 monooxygenase and 3-ketosteroid-Δ 1 -dehydrogenase are key enzymes involved in microbial catabolism of sterols. Here,...
