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Affinity Purification of Glycosylphosphatidylinositol-anchored Proteins by Alpha-Toxin
The glycosylphosphatidylinositol (GPI)-anchor modification attaches a lipid anchor to the C-terminus of a protein, tethering the protein to the cell...
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Affinity-matured HLA class II dimers for robust staining of antigen-specific CD4+ T cells
Peptide–major histocompatibility complex (pMHC) multimers enable the detection of antigen-specific T cells in studies ranging from vaccine efficacy...
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Protein Interaction Analysis by Surface Plasmon Resonance
Surface plasmon resonance (SPR) is an optical technique that is utilized for detecting molecular interactions that occur in direct protein-protein...
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Large Complexes: Cloning Strategy, Production, and Purification
With few exceptions—such as myxobacteria, filamentous cyanobacteria, and actinomycetes (Rokas, Annu Rev Genet 42:235–251, 2008)—bacteria are defined...
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Purification of the Na+-Driven PomAB Stator Complex and Its Analysis Using ATR-FTIR Spectroscopy
The flagellar motor of marine Vibrio is driven by the sodium-motive force across the inner membrane. The stator complex, consisting of two membrane...
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Screening of Recombinant Lignocellulolytic Enzymes Through Rapid Plate Assays
In the search for novel biomass-degrading enzymes through mining microbial genomes, it is necessary to apply functional tests during high-throughput...
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Measuring Mitotic Spindle and Microtubule Dynamics in Marine Embryos and Non-model Organisms
During eukaryotic cell divisionCell division a microtubule-based structure, the mitotic spindle, aligns and segregates chromosomes between daughter...
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Purification, Reconstitution, and Functional Analysis of Connexin Hemichannels
Connexins are the proteins that form the gap junction channels that are essential for cell-to-cell communication. These channels are formed by...
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Substitution of arginine 219 by glycine compromises stability, dimerization, and catalytic activity in a G6PD mutant
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is one of the most common enzymopathies in humans, present in approximately half a billion people...
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High-Throughput Production and Optimization of Membrane Proteins After Expression in Mammalian Cells
High-quality protein samples are an essential requirement of any structural biology experiment. However, producing high-quality protein samples,...
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Thromboxane A2 G Protein-Coupled Receptor Production and Crystallization for Structure Studies
G protein-coupled receptors (GPCRs) play vital roles in human physiology and pathophysiology. This makes the elucidation of the high-resolution...
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Determining Protein Structures Using X-Ray Crystallography
X-ray crystallography is a robust and widely used technique that facilitates the three-dimensional structure determination of proteins at an atomic...
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Heterologous Expression and Purification of GPCRs
G protein-coupled receptors (GPCRs) are involved in a variety of human physiological processes and are attractive targets for treating various...
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Coupling enzymatic activity and gating in an ancient TRPM chanzyme and its molecular evolution
Channel enzymes represent a class of ion channels with enzymatic activity directly or indirectly linked to their channel function. We investigated a...
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Multi-omics reveals new links between Fructosamine-3-Kinase (FN3K) and core metabolic pathways
Fructosamine-3-kinases (FN3Ks) are a conserved family of repair enzymes that phosphorylate reactive sugars attached to lysine residues in peptides...
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Bifunctional and monofunctional α-neoagarooligosaccharide hydrolases from Streptomyces coelicolor A3(2)
AbstractAgar is a galactan and a major component of the red algal cell wall. Agar is metabolized only by specific microorganisms. The final step of...
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NMR Analysis of the Interactions and Conformational Plasticity of Dynein Intermediate Chain
Cytoplasmic dynein complexes play crucial roles in intracellular transport of cellular organelles. While the motor domain of dynein is well...
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Production of Copepod Luciferases via Baculovirus Expression System
Secreted copepod luciferases (CopLucs) represent highly homologous enzymes which catalyze the oxidation of a low molecular weight substrate,...
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Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants
We designed a protein biosensor that uses thermodynamic coupling for sensitive and rapid detection of neutralizing antibodies against severe acute...
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Phase separation propensity of the intrinsically disordered AB region of human RXRβ
RXRβ is one of three subtypes of human retinoid X receptor (RXR), a transcription factor that belongs to the nuclear receptor superfamily. Its...
