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The MBS microbial rapid detection system for rapid detection of major pathogenic bacteria in feed: comparison with plate counting method
The current methods for detecting pathogenic bacteria in feed require high technique and take a long time. The Micro Biological Survey (MBS) rapid...
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A sensitive one-pot ROA assay for rapid miRNA detection
MicroRNAs (miRNAs) and short RNA fragments (18–25 nt) are crucial biomarkers in biological research and disease diagnostics. However, their accurate...
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Rapid detection of fluoroquinolone resistance in Mycobacterium tuberculosis using a novel multienzyme isothermal rapid assay
Simple, rapid, and accurate detection of Fluoroquinolone (FQ) resistance is essential for early initiation of appropriate anti-tuberculosis treatment...
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Rapid Detection Method of Microcystin in Water
Microcystins (MCs) are secondary metabolites produced by some cyanobacteria, a class of cyclic heptapeptide toxins that are stable in the... -
Rapid, tunable, and multiplexed detection of RNA using convective array PCR
Detection of RNA targets is typically achieved through RT-qPCR or RNAseq. RT-qPCR is rapid but limited in number and complexity of targets detected,...
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Rapid detection of tomato spotted wilt virus by real-time RT-LAMP and in-field application
Tomato spotted wilt virus (TSWV) is considered one of the most threatening viruses worldwide for different economically important agricultural crops....
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Establishment of RT-RPA-Cas12a assay for rapid and sensitive detection of human rhinovirus B
Human rhinovirus B (HRV-B) is a major human viral pathogen that can be responsible for various kinds of infections. Due to the health risks...
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Development of fluorescence-linked immunosorbent assay for rapid detection of Staphylococcus aureus
AbstractStaphylococcus aureus is a major pathogen that causes infections and life-threatening diseases. Although antibiotics, such as methicillin,...
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Novel, rapid, and sensitive colorimetric detection of leucomalachite green using a specific aptamer
Leucomalachite green (LMG), a derivative antimicrobial agent, can accumulate in animal tissues for long periods and is categorized as a carcinogen...
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Rapid detection of porcine sapelovirus by reverse transcription recombinase polymerase amplification assay
BackgroundPorcine Sapelovirus (PSV) infection has been confirmed in pigs worldwide, mostly asymptomatic, but in some cases, it can lead to...
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A rapid visual detection method for Potato virus Y based on loop mediated isothermal amplification (LAMP)
In order to establish a rapid and sensitive LAMP visual detection method for Potato virus Y on-site, this study used the conserved fragment of PVY CP ...
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Development of a loop-mediated isothermal amplification (LAMP) assay for rapid visual detection of snakehead vesiculovirus (SHVV) in snakehead
Infections caused by snakehead vesiculovirus (SHVV) have seen frequent outbreaks in recent years, inflicting significant losses on the snakehead...
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Development of a Rapid Detection Method to Prorocentrum lima by Loop-Mediated Isothermal Amplification with Hydroxy Naphthol Blue
Prorocentrum lima , a widely distributed dinoflagellate known for its production of harmful biotoxins, poses a significant threat to humans,...
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A recombinase polymerase amplification–SYBR Green I assay for the rapid and visual detection of Brucella
Brucellosis is a zoonosis caused by Brucella , which poses a great threat to human health and animal husbandry. Pathogen surveillance is an important...
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Rapid detection of virulence-related genes by multiplex PCR in five pathogenic bacteria of mulberry bacterial wilt
Mulberry bacterial wilt is a devastating disease that is difficult to control and causes serious economic losses to the sericulture industry. This...
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The rapid detection of procalcitonin in septic serum using immunoaffinity MALDI chips
BackgroundSepsis is a common worldwide health condition with high mortality. It is caused by a dysregulated immune response to the pathogen. Severe...
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Rapid and specific detection of Chinook salmon bafinivirus (CSBV) in flatfish using loop-mediated isothermal amplification (LAMP)
It is now understood that the flatfish farming industry faces significant threats from Chinook salmon bafinivirus (CSBV) induced hemorrhagic disease....
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Ultra-rapid somatic variant detection via real-time targeted amplicon sequencing
Molecular markers are essential for cancer diagnosis, clinical trial enrollment, and some surgical decision making, motivating ultra-rapid,...
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Development of reverse transcriptase-recombinase polymerase amplification (RT-RPA) assay for rapid detection of large cardamom chirke virus
Large cardamom chirke virus (LCCV) causing chirke disease of large cardamom is a major production constraint of this crop. Rapid and accurate...
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Rapid visual nucleic acid detection of Vibrio alginolyticus by recombinase polymerase amplification combined with CRISPR/Cas13a
Vibrio alginolyticus (V. alginolyticus) is a common pathogen in the ocean. In addition to causing serious economic losses in aquaculture, it can also...