Abstract
In order to establish a rapid and sensitive LAMP visual detection method for Potato virus Y on-site, this study used the conserved fragment of PVY CP gene sequence as a template to design primers, and the rapid visual LAMP detection method of Potato virus Y was successfully established by optimizing the reaction system components (concentration ratio of internal and external primers, and concentrations of loop primers, Bst DNA, Mg2+, dNTP and betaine) and reaction conditions (temperature). The established rapid visual LAMP detection method for Potato virus Y can specifically identify Potato virus Y, providing complete visual detection within 40 min, and the detection limit can reach 4.34 pg μL−1 (cDNA) concentration, which is 100 times more sensitive than PCR. An analysis of 50 potato field samples showed that the results of rapid visualization LAMP and RT-PCR were basically the same. This method is suitable for the rapid detection of Potato virus Y on-site, and this study can also provide theoretical reference for the early diagnosis of other potato viruses.
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The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgements
This research was funded by the Projects of Qinghai Provincial Science and Technology Department (2023-ZJ-724). We are also grateful to the anonymous reviewers for their valuable suggestions and comments.
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JW conceived the idea of the study; YM and YZ analyzed most of the data and interpreted the results; HZ revised the article and interpreted the results.
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Ma, Y., Zhang, Y., Zhu, H. et al. A rapid visual detection method for Potato virus Y based on loop mediated isothermal amplification (LAMP). J Plant Dis Prot 130, 1423–1431 (2023). https://doi.org/10.1007/s41348-023-00786-w
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DOI: https://doi.org/10.1007/s41348-023-00786-w