Abstract
Microcystins (MCs) are secondary metabolites produced by some cyanobacteria, a class of cyclic heptapeptide toxins that are stable in the environment. Microcystins can create various adverse health effects in humans, animals, and plants through contaminated water. Global occurrence and concern about microcystin contamination in water have prompted the development of a range of detection methods for their identification and quantification. However, most protocols are relatively time-consuming and expensive and require laboratory expertise. The development of the inhibition assays and biosensor protocols offered rapid and accurate detection, high reproducibility, and portability. This chapter incorporates the protocols of Protein Phosphatase Inhibition Assay (PPIA), Surface Plasmon Resonance (SPR) immunosensors, Fluorescent immunosensors, Electrochemical Biosensors, and Lateral flow dipstick (Immunostrip) as methods used for MCs detection in terms of their novel development and usage.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Bittencourt-Oliveira MC, Oliveira MC, Pinto E (2011) Diversidade de genótiposprodutores de microcistinasemlinhagensbrasileiras de microcystis (Cyanobacteria). Braz J Biol 71:209–216
Carmichael WW (2001) Health effects of toxin-producing cyanobacteria: “the CyanoHABs”. Hum Ecol Risk Assess Int J 7(5):1393–1407
Falconer IR, Burch MD, Steffensen DA, Choice M, Coverdale OR (1994) Toxicity of the blue-green alga (cyanobacterium) Microcystis aeruginosa in drinking water to growing pigs as an animal model for human injury and risk assessment. Environ Toxicol Water Qual 9(2):131–139
Lawton LA, Edwards C (2008) Conventional laboratory methods for cyanotoxins. In: Cyanobacterial harmful algal blooms: state of the science and research needs. Springer, New York, NY, pp 513–537
McElhiney J, Lawton LA (2005) Detection of the cyanobacterial hepatotoxins microcystins. Toxicol Appl Pharmacol 203(3):219–230
McElhiney J, Lawton LA, Porter AJ (2000) Detection and quantification of microcystins (cyanobacterial hepatotoxins) with recombinant antibody fragments isolated from a naive human phage display library. FEMS Microbiol Lett 193(1):83–88
Pang P, Lai Y, Zhang Y, Wang H, Conlan XA, Barrow CJ, Yang W (2020) Recent advancement of biosensor technology for the detection of microcystin-LR. Bull Chem Soc Jpn 93(5):637–646
Qin J, Sun X, Li D, Yan G (2019) Phosphorescent immunosensor for simple and sensitive detection of microcystin-LR in water. RSC Adv 9(22):12747–12754
Singh S, Srivastava A, Oh HM, Ahn CY, Choi GG, Asthana RK (2012) Recent trends in the development of biosensors for the detection of microcystin. Toxicon 60(5):878–894
Strachan IB, Pattey E, Boisvert JB (2002) Impact of nitrogen and environmental conditions on corn as detected by hyperspectral reflectance. Remote Sens Environ 80(2):213–224
Ueno, Y., Nagata, S., Tsutsumi, T., Hasegawa, A., Watanabe, M. F., Park, H. D., et al (1996) Detection of microcystins, a blue-green algal hepatotoxin, in drinking water sampled in Haimen and Fusui, endemic areas of primary liver cancer in China, by highly sensitive immunoassay
WHO (1998) Guidelines for drinking water quality addendum to Vol. 2. Health criteria and other supporting information, 2nd edn. World Health Organisation, Geneva, pp 95–110
Yang R, Song D, Fang S, Liu Y, Zhou X, Long F, Zhu A (2018) Development of novel portable and reusable fibre optical chemiluminescent biosensor and its application for sensitive detection of microcystin-LR. Biosens Bioelectron 121:27–33
Zhou L, Yu H, Chen K (2002) Relationship between microcystin drinking water and colorectal cancer. Biomed Environ Sci 15:166–171
Acknowledgment
The authors wish to thank the University of Sri Jayewardenepura.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.
About this chapter
Cite this chapter
Liyanage, G.Y., Manage, S.P., Abeysiri, H.A.S.N., Manage, P.M., Kulasooriya, S.A. (2023). Rapid Detection Method of Microcystin in Water. In: Thajuddin, N., Sankara narayanan, A., Dhanasekaran, D. (eds) Protocols for Cyanobacteria Sampling and Detection of Cyanotoxin . Springer, Singapore. https://doi.org/10.1007/978-981-99-4514-6_50
Download citation
DOI: https://doi.org/10.1007/978-981-99-4514-6_50
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-99-4513-9
Online ISBN: 978-981-99-4514-6
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)